Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): Homo sapiens

PROVIDER: GSE294012 | GEO | 2025/04/08

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [scRNA-seq]

Project description:To establish an in vitro endometrial co-culture model, endometrial organoid was co-cultured with endometrial stromal cells in a 3D hydrogel matrix. During this co-culture process, tubular gland development was identified from endometrial organoids, which showed the molecular, morphological and functional properties of human endometrial glands. To further characterize the genetic drive for this phenomenon, we conducted single-cell RNA sequencing analysis at day 1 and day 9 after co-culture.

2025-03-29 | GSE292827 | GEO

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [bulk RNA-seq]

Project description:Little is known about the adenogenesis of human endometrial glands. This study explored endometrial adenogenesis using a novel three-dimensional endometrial assembloid model that integrates human endometrial organoids (EOs) and human endometrial stromal cells (HESCs). The model effectively recapitulated endometrial tubular gland formation, underscoring the critical role of stromal-epithelial interactions. Transcriptomic analyses identified WNT7B as a key intrinsic regulator for EO-derived tubular gland formation, which is extrinsically regulated by TGFβ1-VDR interaction between HESCs and EOs. Estradiol stimulated endometrial gland development via WNT7B downregulation in EOs. This finding was validated in an estradiol-stimulated mouse model and clinical samples from women undergoing in vitro fertilization (IVF) cycles. Uterine-specific WNT7B knockout in mice further confirmed its inhibitory role in endometrial gland development. This study offers insights into endometrial adenogenesis and potential therapeutic targets for related endometrial disorders.

2025-04-08 | GSE294011 | GEO

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression

Project description:Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression

| PRJNA1247687 | ENA

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [scRNA-seq]

Project description:Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [scRNA-seq]

| PRJNA1241322 | ENA

Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [bulk RNA-seq]

Project description:Endometrial assembloid model reveals endometrial adenogenesis regulation by estradiol-driven WNT7B expression [bulk RNA-seq]

| PRJNA1247690 | ENA

Endometrial Cancer Onset is Driven by a Specific Epimutation Suppressing PAX2.

Project description:This SuperSeries is composed of the SubSeries listed below.

2025-06-03 | GSE275345 | GEO

Single-cell RNA Sequencing of Endometrial Assembloid Cultures

Project description:Decidual remodelling of midluteal endometrium leads to a short implantation window after which the uterine mucosa either breaks down or is transformed into a robust matrix that accommodates the placenta throughout pregnancy. To gain insights into the underlying mechanisms, we established and characterised endometrial assembloids, consisting of gland organoids and primary stromal cells. Single-cell transcriptomics revealed that decidualized assembloids closely resemble midluteal endometrium, harbouring differentiated and senescent subpopulations in both glands and stroma. We show that acute senescence in glandular epithelium drives secretion of multiple canonical implantation factors, whereas in the stroma it calibrates the emergence of anti-inflammatory decidual cells and pro-inflammatory senescent decidual cells. Pharmacological inhibition of stress responses in pre-decidual cells accelerated decidualization by inhibiting senescence and mesenchymal-epithelial transition, processes involved in endometrial breakdown and regeneration, respectively. Accelerated decidualization resulted in entrapment of co-cultured human blastocysts in a largely static decidual matrix. By contrast, the presence of senescent decidual cells created a dynamic implantation environment, enabling embryo expansion and attachment, although their persistence led to gradual disintegration of assembloids. Together, our findings demonstrate that senescence controls endometrial fate decisions at implantation and highlight how endometrial assembloids may accelerate the discovery of new treatments to prevent reproductive failure.

2021-03-08 | GSE168405 | GEO

A molecular staging model for accurately dating the endometrial biopsy

Project description:This SuperSeries is composed of the SubSeries listed below.

2023-09-06 | GSE234434 | GEO

Aging of mouse spermatogonial stem cells by Wnt7b-Jnk pathway activation

Project description:This SuperSeries is composed of the SubSeries listed below.

2019-07-30 | GSE120778 | GEO

Tumor suppressor role of Ezh2 in an NRASQ61K driven model of Early T-cell Precursor Acute Lymphoblastic Leukemia

Project description:This SuperSeries is composed of the SubSeries listed below. Refer to individual Series

2016-01-07 | E-GEOD-76603 | biostudies-arrayexpress