Dual-Color MicroRNA Array Analysis Reveals Compartment-Specific Differential Expression in Diabetic vs. Normal DBA/2J Mouse Kidney Cortex and Medulla

ABSTRACT: This study profiles microRNA expression in mouse kidney tissues comparing diabetic and normal conditions. A total of 16 samples from the cortex and medulla compartments were analyzed from male DBA/2J mice (6–8 weeks old). RNA quality was rigorously assessed, followed by labeling with the miRCURY LNA™ microRNA Hi-Power Labeling Kit. In a dual-color experimental setup, the sample RNA was labeled with Cy3, and a Cy5-labeled reference was used. Arrays were hybridized under automated conditions, scanned with an Agilent G2565BA Microarray Scanner, and the data were processed using background correction and global Lowess normalization. Differential expression analysis, performed using both t-tests (with Benjamini–Hochberg correction) and ANOVA, identified a subset of microRNAs with significant expression differences between diabetic and normal kidneys.

ORGANISM(S): Mus musculus

PROVIDER: GSE294043 | GEO | 2025/06/10

REPOSITORIES: GEO