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HOMEODOMAIN-LIKE shapes chromatin architecture and rewires leaf epidermal patterns [ChIPseq]

ABSTRACT: During Arabidopsis (Arabidopsis thaliana) leaf epidermis development, precursor cells divide and differentiate to form distinct patterns of trichomes, pavement cells, and stomata. The mechanisms governing these developmental transitions remain unclear. We identified the homeodomain-like protein HDL, which is required for heterochromatin compaction through interactions with the histone deacetylase HDA6 and the methyltransferase KRYPTONITE during the cell cycle. Mutants of HDL showed a prolonged interphase, fewer cell divisions, and greater endoreduplication, resulting in fewer stomata and more trichomes. Chromatin immunoprecipitation and ATAC-sequencing revealed higher chromatin accessibility in HDL-bound regions and lower chromatin accessibility along the promoters of stomata-related genes, which also showed reduced expression in hdl mutants. These findings highlight the importance of chromatin organization in maintaining cell division potential and transcriptional control.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE294312 | GEO | 2026/07/29

REPOSITORIES: GEO

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HOMEODOMAIN-LIKE shapes chromatin architecture and rewires leaf epidermal patterns [RNA-seq]

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