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A strategy for challenging tumorous bone regeneration by utilizing borosilicate bioactive glass synchronously boosting osteosarcoma combating and osteogenesis promotion of moderate magnetic hyperthermia

ABSTRACT: To investigate the relevant biological mechanism involved in the inhibition of 143B cells when cultured with injectable magnetothermal bioactive systems (IMBSs) under alternating magnetic field (AMF), the high-throughput RNA sequencing was conducted.

ORGANISM(S): Homo sapiens

PROVIDER: GSE294322 | GEO | 2025/04/20

REPOSITORIES: GEO

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A strategy for challenging tumorous bone regeneration by utilizing borosilicate bioactive glass synchronously boosting osteosarcoma combating and osteogenesis promotion of moderate magnetic hyperthermia

Project description:To investigate the relevant biological mechanism involved in the osteogenic differentiation of human bone marrow mesenchymal stem cells (hBMSCs) when cultured with BSG extracts under moderate hyperthermia (42℃, 5%CO2）or regular atmosphere (37℃, 5%CO2 ), the high-throughput RNA sequencing was conducted.

2025-04-20 | GSE294487 | GEO

Project description:A strategy for challenging tumorous bone regeneration by utilizing borosilicate bioactive glass synchronously boosting osteosarcoma combating and osteogenesis promotion of moderate magnetic hyperthermia

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Magnetic mesoporous bioactive glass for synergetic use in bone regeneration, hyperthermia treatment, and controlled drug delivery.

Project description:A combination of chemotherapy with hyperthermia can produce remarkable success in treating advanced cancers. For this purpose, magnetite (Fe3O4)-doped mesoporous bioactive glass nanoparticles (Fe3O4-MBG NPs) were synthesized by the sol-gel method. Fe3O4-MBG NPs were found to possess spherical morphology with a size of approximately 50 ± 10 nm and a uniform pore size of 9 nm. The surface area (309 m2 g-1) was sufficient for high drug loading capacity and mitomycin C (Mc), an anticancer drug, was entrapped in the Fe3O4-MBG NPs. A variable rate of drug release was observed at different pH values (6.4, 7.4 & 8.4) of the release media. No significant death of normal human fibroblast (NHFB) cells was observed during in vitro analysis and for Mc-Fe3O4-MBG NPs considerable inhibitory effects on the viability of cancer cells (MG-63) were observed. When Fe3O4-MBG NPs were immersed in simulated body fluid (SBF), hydroxycarbonate apatite (HCA) was formed, as confirmed by XRD and FTIR spectra. A negligible value of coercivity and zero remanence confirms that Fe3O4-MBG NPs are superparamagnetic. Fe3O4-MBG NPs showed a hyperthermia effect in an alternating magnetic field (AMF), and a rise of 11.5 °C in temperature during the first 6 min, making it suitable for hyperthermia applications. Fe3O4-MBG NPs expressed excellent biocompatibility and low cytotoxicity, therefore, they are a safe biomaterial for bone tissue regeneration, drug delivery, and hyperthermia treatment.

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Multifunctional temozolomide-loaded lipid superparamagnetic nanovectors: dual targeting and disintegration of glioblastoma spheroids by synergic chemotherapy and hyperthermia treatment

Project description:The recent develop of nanotechnology promises to revolutionize the delivery of chemotherapeutic agents across the blood-brain barrier and against cancer cells. Superparamagnetic iron oxide nanoparticles have been successfully exploited in many different clinical trials for the remote hyperthermal treatment of cancer cells in response to alternated magnetic fields (AMF) and as nontargeted contrast agents for magnetic resonance imaging (MRI). In this work, the functionalization of SPIONs- and TMZ-loaded lipid magnetic nanovectors (LMNVs) with the antibody against the transferrin receptor (TfR) for the dual targeting of the endothelial cells of BBB and GBM cells is reported. The targeting efficiency of the functionalized nanovectors (AbLMNVs) has been demonstrated on a multicellular organoid system modeling the BBB and the microscopic GBM foci. Transcytosis through endothelial cells and penetration in GBM spheroids of functionalized nanovectors have been verified and quantified through flow cytometry analysis and different imaging techniques. Moreover, the lipid component of the functionalized nanovectors has been modified with a lipophilic temperature sensitive fluorescent dye to monitor the intraparticle temperature in response to AMF Chronic AMF treatments of microscopic GBM spheroids targeted with the functionalized nanovectors, plain or loaded with TMZ drug, were carried out and their elevated potential to induce spheroid disintegration, cell necrosis and apoptosis was revealed. Finally, magnetothermal ability of nanovectors was successfully tested on a post-mortem brain tissue.

2019-11-25 | PXD014652 | Pride

Bioactive Glass Nanoparticles for Tissue Regeneration.

Project description:Sol-gel-derived bioactive glass nanoparticles have attracted special interest due to their potential as novel therapeutic and regenerative agents. Significant challenges are yet to be addressed. The fabrication of sol-gel-derived nanoparticles in binary and ternary systems with an actual composition that meets the nominal has to be achieved. This work addresses this challenge and delivers nanoparticles in a ternary system with tailored composition and particle size. It also studies how specific steps in the fabrication process can affect the incorporation of the metallic ions, nanoparticle size, and mesoporosity. Sol-gel-derived bioactive glass nanoparticles in the 62 SiO2-34.5 CaO-3.2 P2O5 (mol %) system have been fabricated and characterized for their structural, morphological, and elemental characteristics using Fourier transform infrared spectroscopy, X-ray diffraction analysis, scanning electron microscopy associated with elemental analysis, transmission electron microscopy, and solid-state nuclear magnetic resonance. The fabricated nanoparticles were additionally observed to form the apatite phase when immersed in simulated body fluid. This work highlights the effect of the different processing variables, such as the nature of the solvent, the order in which reagents are added, stirring time, and the concentrations in the catalytic solution on the controlled incorporation of specific ions (e.g., P and Ca) in the nanoparticle network and particle size.

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Enhanced bioactive properties of BiodentineTM modified with bioactive glass nanoparticles.

Project description:To prepare nanocomposite cements based on the incorporation of bioactive glass nanoparticles (nBGs) into BiodentineTM (BD, Septodent, Saint-Maur-des-Fosses Cedex, France) and to assess their bioactive properties.nBGs were synthesised by the sol-gel method. BD nanocomposites (nBG/BD) were prepared with 1 and 2% nBGs by weight; unmodified BD and GC Fuji IX (GIC, GC Corporation, Tokyo, Japan) were used as references. The in vitro ability of the materials to induce apatite formation was assessed in SBF by X-ray diffraction (XRD), attenuated total reflectance with Fourier transform infrared spectroscopy (ATR-FTIR), and scanning electron microscopy (SEM) with energy dispersive X-ray (EDX) analysis. BD and nBG/BD were also applied to dentine discs for seven days; the morphology and elemental composition of the dentine-cement interface were analysed using SEM-EDX.One and two percent nBG/BD composites accelerated apatite formation on the disc surface after short-term immersion in SBF. Apatite was detected on the nBG/BD nanocomposites after three days, compared with seven days for unmodified BD. No apatite formation was detected on the GIC surface. nBG/BD formed a wider interfacial area with dentine than BD, showing blockage of dentine tubules and Si incorporation, suggesting intratubular precipitation.The incorporation of nBGs into BD improves its in vitro bioactivity, accelerating the formation of a crystalline apatite layer on its surface after immersion in SBF. Compared with unmodified BD, nBG/BD showed a wider interfacial area with greater Si incorporation and intratubular precipitation of deposits when immersed in SBF.

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Boosting the effects of hyperthermia-based anticancer treatments by HSP90 inhibition.

Project description:Hyperthermia - application of supra-physiological temperatures to cells, tissues or organs - is a pleiotropic treatment that affects most aspects of cellular metabolism, but its effects on DNA are of special interest in the context of cancer research and treatment. Hyperthermia inhibits repair of various DNA lesions, including double-strand breaks (DSBs), making it a powerful radio- and chemosensitizer, with proven clinical efficacy in therapy of various types of cancer, including tumors of head and neck, bladder, breast and cervix. Among the challenges for hyperthermia-based therapies are the transient character of its effects, the technical difficulties in maintaining uniformly elevated tumor temperature and the acquisition of thermotolerance. Approaches to reduce or eliminate these challenges could simplify the application of hyperthermia, boost its efficacy and improve treatment outcomes. Here we show that a single, short treatment with a relatively low dose of HSP90 inhibitor Ganetespib potentiates cytotoxic as well as radio- and chemosensitizing effects of hyperthermia and reduces thermotolerance in cervix cancer cell lines. Ganetespib alone, applied at this low dose, has virtually no effect on survival of non-heated cells. Our results thus suggest that HSP90 inhibition can be a safe, simple and efficient approach to improving hyperthermia treatment efficacy and reducing thermotolerance, paving the way for in vivo studies.

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Impact of Glass Composition on Hydrolytic Degradation of Polylactide/Bioactive Glass Composites.

Project description:Understanding the degradation of a composite material is crucial for tailoring its properties based on the foreseen application. In this study, poly-L,DL-lactide 70/30 (PLA70) was compounded with silicate or phosphate bioactive glass (Si-BaG and P-BaG, respectively). The composite processing was carried out without excessive thermal degradation of the polymer and resulted in porous composites with lower mechanical properties than PLA70. The loss in mechanical properties was associated with glass content rather than the glass composition. The degradation of the composites was studied for 40 weeks in Tris buffer solution Adding Si-BaG to PLA70 accelerated the polymer degradation in vitro more than adding P-BaG, despite the higher reactivity of the P-BaG. All the composites exhibited a decrease in mechanical properties and increased hydrophilicity during hydrolysis compared to the PLA70. Both glasses dissolved through the polymer matrix with a linear, predictable release rate of ions. Most of the P-BaG had dissolved before 20 weeks in vitro, while there was still Si-BaG left after 40 weeks. This study introduces new polymer/bioactive glass composites with tailorable mechanical properties and ion release for bone regeneration and fixation applications.

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Ground-glass opacities: a problem bound to get more challenging.

Project description: Not available

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