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Expression data from human cord blood CD34+ CD38- cells

ABSTRACT: We used microarrays to observe the global gene expression in hematopoietic stem and projenitor cells during ex vivo culture with DMSO (Blank) or with Garcinol (GAR) and identified distinct classes of up or down-regulated genes.

ORGANISM(S): Homo sapiens

PROVIDER: GSE29459 | GEO | 2012/06/01

SECONDARY ACCESSION(S): PRJNA141579

REPOSITORIES: GEO

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Expression data from human cord blood CD34+ CD38- cells

Project description:We used microarrays to observe the global gene expression in hematopoietic stem and projenitor cells during ex vivo culture with DMSO (Blank) or with Garcinol (GAR) and identified distinct classes of up or down-regulated genes. CD34+ cells were cultured ex vivo with DMSO (Blank) or with Garcinol (GAR) for 7 days. CD34+ CD38- cells were sorted from cultured cells (blank and GAR) with a FACS and total RNA were extracted from the sorted cells (blank and GAR), respectively. Blank represents a control sample.

2012-05-31 | E-GEOD-29459 | biostudies-arrayexpress

Wnt1 Accelerates an Ex Vivo Expansion of Human Cord Blood CD34(+)CD38(-) Cells.

Project description: Not available

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Phenotyping and Target Expression Profiling of CD34+/CD38- and CD34+/CD38+ Stem- and Progenitor cells in Acute Lymphoblastic Leukemia.

Project description: Not available

| S-EPMC5994777 | biostudies-literature

Delineation of target expression profiles in CD34+/CD38- and CD34+/CD38+ stem and progenitor cells in AML and CML.

Project description: Not available

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Transcription profiling of CD34+CD38- vs CD34+CD38+ fractions of human umbilical cord blood and comparison of slow-dividing fraction vs the fast dividing fraction of CD34+/CD38- population

Project description:Comparison of the CD34+CD38- versus CD34+CD38+ fractions of human umbilical cord blood and comparison of the slow-dividing fraction versus the fast dividing fraction of the CD34+/CD38- population.

2005-09-08 | E-EMBL-1 | biostudies-arrayexpress

Transcriptional changes due to the [GAR+] prion

Project description:Comparison between [gar-] and [GAR+] samples of the W303 background grown in 2% glucose. Three separate [gar-] and [GAR+] cultures were grown to late exponential phase (OD600~0.8) prior to phenol-chloroform extraction. Biological replicates are [gar-] samples #1, 2, and 3 and [GAR+] samples #1, 2, and 3. [gar-] and [GAR+] sample sets were compared to determine transcriptional differences.

2009-07-01 | E-GEOD-12479 | biostudies-arrayexpress

Expression data from CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cellsmbryo

2008-09-10 | GSE6146 | GEO

RNA-seq analysis of freshly isolated and ex-vivo cultured human cord blood CD34+ cells

Project description:RNA-seq analysis was performed to compare differentially expressed genes in freshly isolated and ex-vivo cultured human cord blood CD34+ cells. Mitochondrion related genes are upregulated in CD34+ hematopoietic stem and progenitor cells upon ex vivo culture. In vivo transplantation experiments demonstrate that stemness of CD34+ cells is significantly decreased due to oxidative stress induced by ex vivo culture.

2021-09-30 | GSE179928 | GEO

Prostaglandin-modulated umbilical cord blood hematopoietic stem cells

Project description:Umbilical cord blood (UCB) is a valuable source of hematopoietic stem cells (HSCs) for use in allogeneic transplantation. Key advantages of UCB are rapid availability and less stringent requirements for HLA matching. However, UCB contains an inherently limited HSC count, which is associated with delayed time to engraftment, high graft failure rates and early mortality. 16,16 dimethyl prostaglandin E2 (dmPGE2) was previously identified to be a critical regulator of HSC homeostasis and we hypothesized that a brief ex vivo modulation could improve patient outcomes by increasing the “effective dose” of HSCs. Molecular profiling with Affymetrix GeneChips were used to determine the optimal ex vivo modulation conditions (e.g., temperature and media) for use in a clinical setting by measured pathway induced expression changes. Isolated human CD34+ from umbilical cord blood were incubated ex vivo in Stem Span (SS) media evaluating three treatment temperatures (4 deg C, 25 deg C, and 37 deg C) with 10uM 16,16-dmPGE2 or Vehicle (DMSO) for 2 hours. To evaluate optimal media, similar CD34+ cells were incubated ex vivo in either Stem Span-SFEM (SS) media or 8% Low Molecular Weigh Dextran 40/5% HSA solution (LMD/HSA) with 10uM 16,16-dmPGE2 or Vehicle (DMSO) for 2 hours at 37 deg C. Total RNA was isolated post incubation and analyzed on Affymetrix microarrays for pathway activation.

2013-09-12 | E-GEOD-46569 | biostudies-arrayexpress

Transcription profiling of CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cells

Project description:Gene expression profiles of CD34+CD38- stem cells and more differentiated CD34+CD38+ progenitor cells were compared. Comparison of expression profiles of hematopoietic stem cells from fetal liver, umbilical cord blood, bone marrow and mobilized pheripheral blood allowed us to identify a unique set of genes with conserved expression during ontogeny. Experiment Overall Design: CD34+CD38- en CD34+CD38+ cell populations were isolated by cell sorting from human Bone Marrow, mobilized peripheral blood, umbilical cord blood and fetal liver. Total RNA was isolated from each cell population followed by the synthesis of biotinylated cRNA. After fragmentation the biotinylated cRNA was hybridized to affymetrix U133A chips.

2008-10-25 | E-GEOD-6146 | biostudies-arrayexpress

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