Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Cellular NAD+ availability constrains developmental speed in the Drosophila eye

ABSTRACT: The cellular and biochemical processes that limit the rates at which embryos develop, tissues form, and cells differentiate remain largely unknown. Using the speed of progression of a differentiation front in the developing eye of Drosophila as a proxy for developmental speed, we identified genetic perturbations that slowed down the progression of this front. We found that inhibition of the Electron Transport Chain (ETC), and more generally of energy production in mitochondria, reduced developmental speed, led to increased NADH/ NAD+ ratio but did not change ATP levels due to a compensatory increase in glycolysis. Using targeted perturbations, we further showed that the metabolic state of cells located at and ahead of the differentiation front determined the speed of this front. Additionally, increasing the rate of regeneration of the NAD+ pool suppressed the defects resulting from the inhibition of the ETC whereas inhibition of the NAD+ salvage pathway led to reduced developmental speed. Thus, developmental speed appeared to be limited by the demand for NAD+, at least upon reduced energy supply.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE294725 | GEO | 2026/02/25

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Cellular NAD+ availability constrains developmental speed in the Drosophila eye

Project description:Cellular NAD+ availability constrains developmental speed in the Drosophila eye

| PRJNA1251508 | ENA

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development [mTORi]

Project description:Embryos from different mammalian species develop at characteristic timescales. These timescales are recapitulated during the differentiation of pluripotent stem cells in vitro. Specific genes and molecular pathways that modulate cell differentiation speed between mammalian species remain to be identified. Here we use single-cell multi-omic analysis of neural differentiation of mouse, cynomolgus monkey and human pluripotent cells to identify new regulators for differentiation speed. We demonstrate that species-specific differences in transcriptome dynamics are mirrored at the chromatin level, but that, contrary to other developmental contexts, the speed of neural differentiation is insensitive to manipulations of cell growth and cycling. Exploiting the single-cell resolution of our data, we identify glycogen storage levels regulated by UGP2 expression as a new species-specific trait of pluripotent cells, and show that lowered glycogen storage in UGP2 mutant cells is associated with accelerated neural differentiation. The control of energy storage could be a general strategy for the regulation of cell differentiation speed.

2024-09-05 | GSE275571 | GEO

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development [UGP2 KO]

2024-09-05 | GSE275566 | GEO

Single-cell multiome uncovers differences in glycogen metabolism underlying species-specific speed of development [scRNAseq and scATACseq]

2024-09-05 | GSE276058 | GEO

Lactate deficiency-induced early embryonic arrest is mitigated through malate-aspartate shuttle

Project description:During assisted reproductive technology (ART) procedure, approximately one third to half of the in vitro fertilization (IVF) derived embryos cannot reach the blastocyst stage, and about 10% arrest at the early cleavage stage. Early mammalian embryos obtain nutrients from maternal environment to fulfill the energy requirements of growth and development, and lactate is one of the major substrates of embryo energy metabolism. To explicitly reveal the energy metabolism in early embryogenesis, we inhibited the production and uptake of lactate at the zygote stage, and found it resulted developmental arrest, impaired mitochondrial activity and reduced NAD+/NADH ratio. By activating the malate-aspartate shuttle (MAS), the developmental arrest was rescued through the restoration of NAD+/NADH balance. Therefore, we demonstrate that maternal LDHB plays an essential role from 4- to 8-cell stage that it converts lactate from pyruvate to generate NAD+, and MAS activation is responsible for the re-equilibrium of NAD+/NADH. Our study discusses the association of metabolic flexibility and developmental regulation, and deepens our understanding of the metabolic mechanism of embryonic development.

2025-06-12 | GSE299062 | GEO

Knockout of Hsp70 genes significantly affects locomotion speed and gene expression in leg skeletal muscles of Drosophila melanogaster

Project description:The functions of the Hsp70 genes were studied using a line of D. melanogaster with knockout of six these genes out of thirteen. Namely, effect of knockout of Hsp70 genes on negative geotaxis climbing (locomotor) speed and the ability to adapt to climbing training (0.5-1.5 h/day, 7 days/week, 19 days) were examined. Seven- and 23-day-old Hsp70– flies demonstrated a comparable reduction (2-fold) in locomotor speed and widespread changes in leg skeletal muscle transcriptome (RNA-seq), compared to w1118 flies. To identify the functions of genes related to decreased locomotor speed the overlapped differentially expressed genes at both time points were analyzed: the up-regulated genes encoded extracellular proteins, regulators of drug metabolism and antioxidant response, while down-regulated genes encoded regulators of carbohydrate metabolism and transmembrane proteins. Additionally, in Hsp70– flies, activation of transcription factors related to disruption of the fibril structure and heat shock response (Hsf) were predicted, using the position weight matrix approach. In the control flies, adaptation to chronic exercise training was associated mainly with gene response to a single exercise bout, while the predicted transcription factors were related to stress/immune (Hsf, NF-kB, etc.) and early gene response. In contrast, Hsp70– flies demonstrated no adaptation to training, as well as significantly impaired gene response to a single exercise bout. In conclusion, the knockout of Hsp70 genes not only reduced physical performance, but also disrupted adaptation to chronic physical training, which is associated with changes in leg skeletal muscle transcriptome and impaired gene response to a single exercise bout.

2023-08-01 | GSE239395 | GEO

DNA replication fork speed Acts as A Pacer in Cortical Neurogenesis

Project description:DNA replication fork speed, which controls the rate of genome duplication, has emerged as a key regulator of cellular plasticity. However, its role in neurogenesis remains unexplored. Mini-chromosome maintenance complex (MCMs)-binding protein (MCMBP) functions as a chaperone for newly synthesized MCMs, increasing chromatin coverage to restrain fork speed. We demonstrate that selective deletion of MCMBP in neural progenitor radial glial cells (RGCs) accelerates fork speed, triggering widespread apoptosis, DNA damage, and micronuclei formation, ultimately activating p53 and causing microcephaly. Unexpectedly, concurrent deletion of Trp53 and Mcmbp further increased fork speed, leading to extensive RGC detachment from the ventricular zone and acquisition of outer-RGC characteristics. Mechanistically, we found that the MCM3 subunit coordinates DNA and centrosome duplication, thereby mediating RGC attachment. Behavioral analysis revealed that disruption of fork speed results in anxiety-like behavior in mice. These findings unveil a previously unrecognized role for replication fork speed in neurogenesis.

2025-09-27 | GSE279962 | GEO

Organismal Benefits of Transcription Speed Control at Gene Boundaries

Project description:We modulate RNAPII transcription speed by point mutations in the second largest subunit of Arabidopsis RNAPII, NRPB2. Nascent transcription profiling revealed that accelerated RNAPII complexes clear stalling sites at both gene ends, resulting in read-through transcription. Accelerated nascent RNAPII transcription increased the association with 5'SS intermediates and improved splicing efficiency, supporting tight connections between transcription speed and co-transcriptional pre-RNA processing.

2020-02-14 | GSE133143 | GEO

High-speed fluorescence image-enabled cell sorting

Project description:High-speed fluorescence image-enabled cell sorting

| PRJNA705647 | ENA

Molecular blueprints for spinal circuit modules controlling locomotor speed

Project description:The flexibility of motor actions is ingrained in the diversity of neurons and how they are organized into functional circuit modules, yet our knowledge of the molecular underpinning of motor circuit modularity remains limited. Locomotion is a motor behavior characterized by sudden changes in speed and strength enabled by the coordinated recruitment of different motoneuron subtypes. Here we use adult zebrafish to link the molecular diversity of motoneurons and the rhythm-generating V2a interneurons with their modular circuit organization that is responsible for changes in locomotor speed. We show that the molecular diversity of motoneurons and V2a interneurons reflects their functional segregation into slow, intermediate or fast subtypes. Furthermore, we reveal shared molecular signatures between V2a interneurons and motoneurons of the three speed circuit modules. Overall, by characterizing how the molecular diversity of motoneurons and V2a interneurons relates to their function, connectivity and behavior, our study provides important insights not only into the molecular mechanisms for neuronal and circuit diversity for locomotor flexibility but also for charting circuits for motor actions in general.

2023-09-29 | GSE243993 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data