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Cholinergic neuron-to-glioblastoma synapses in a human iPSC-derived co-culture model

ABSTRACT: This study examines a co-culture model of human iPSC-derived cholinergic neurons with glioblastoma (GBM) cells derived from GBM organoids (GBOs; UP-10072). To examine transcriptional regulation of GBM cells by cholinergic neurons, we performed scRNAseq of a set of distinct conditions: 1) cholinergic neurons only; 2) GBM cells only; 3) GBM cells treated with conditioned medium from neurons; 4) direct GBM cell-cholinergic neuron co-cultures.

ORGANISM(S): Homo sapiens

PROVIDER: GSE294747 | GEO | 2025/05/17

REPOSITORIES: GEO

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