Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptome profiling of SKHEP1 cells and differentiated SKHEP1 cells induced by forced expresssion of MYOD1 using RNA-sequencing.

ABSTRACT: The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. SKHEP1 is a human hepatocellular carcinoma cell line. We found that SKHEP1 cells differentiated into muscle cells upon induction by muscle differentiation factor MYOD1.

ORGANISM(S): Homo sapiens

PROVIDER: GSE295157 | GEO | 2026/06/01

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

Transcriptome profiling of HCT116 cells and differentiated HCT116 cells induced by forced expresssion of MYOD1 or PPARG using RNA-sequencing.

2026-06-01 | GSE295155 | GEO

Transcriptome profiling of CT26 cells and differentiated CT26 cells induced by forced expresssion of Myod1 or Pparg using RNA-sequencing.

2026-06-01 | GSE295153 | GEO

Transcriptome profiling of SKHEP1 cells and differentiated SKHEP1 cells induced by forced expresssion of PPARG using RNA-sequencing.

2026-06-01 | GSE295158 | GEO

Transcriptome profiling of B16F10 cells and differentiated B16F10 cells induced by forced expresssion of Myod1, Pparg, or by knockdown of Setdb1 using RNA-sequencing.

Project description:The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. B16F10 is a mouse melanoma cell line. We found that B16F10 cells differentiated into muscle cells or adipocytes upon induction by muscle or adipocyte differentiation factor Myod1 or Pparg, respectively. Moreover, Setdb1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of Setdb1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. Setdb1 is upregulated in cancer cells and promotes cancers. We found that knockdown of Setdb1 in B16F10 cells led to a neuronal-like differentiation phenotype.

2026-06-01 | GSE295152 | GEO

Transcriptome profiling of SKHEP1 cells and differentiated SKHEP1 cells induced by knockdown of SETDB1 using RNA-sequencing

Project description:The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. SKHEP1 is a human hepatocellular carcinoma cell line. SETDB1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of SETDB1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. SETDB1 is upregulated in cancer cells and promotes cancers. We found that knockdown of SETDB1 in SKHEP1 cells led to a neuronal-like differentiation phenotype.

2026-06-01 | GSE295159 | GEO

Transcriptome profiling of CT26 cells and de-differentiated CT26 cells induced by forced expresssion of Oct4 and SOX2 using RNA-sequencing.

Project description:The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. It has been reported that neural stem cells are uniquely immune-privileged, suggesting that neural stemness also confers cancer cells with the ability of immune evasion or immune privilege. CT26 is a mouse colon cancer cell line. We found that dedifferentiation of CT26 cells with overexpression of Oct4 and SOX2 enhanced neural stemness and decreased immunogenicity in cells.

2026-05-31 | GSE298039 | GEO

Transcriptome profiling of CT26 cells and differentiated CT26 cells induced by knockdown of Setdb1 using RNA-sequencing.

Project description:The core proeprty of cancer cells is neural stemness, which determines tumorigenicity and pluripotency. Therefore, cancer cells can supposedly be induced to differentiate along different lineages. CT26 is a mouse colon cancer cell line. Setdb1 is an epigenetic modification factor responsible for catalyzing the histone modification H3K9me3. Transcription of Setdb1 gene is enriched in embryonic neural cells during vertebrate embryogenesis. Setdb1 is upregulated in cancer cells and promotes cancers. We found that knockdown of Setdb1 in CT26 cells led to a neuronal-like differentiation phenotype.

2026-06-01 | GSE295154 | GEO

Pro-neuronal activity of Myod1 due to promiscuous binding [ATAC-seq]

Project description:Basic helix-loop-helix (bHLH) pioneer transcription factors Myod1 and Ascl1 are biochemically related but produce fundamentally different outcomes when expressed in fibroblasts: Myod1 produces muscle cells and Ascl1 induces neurons. Here, we sought to investigate the molecular mechanisms explaining the differential activity. Surprisingly, we found a large overlap in the overall binding patterns of Ascl1 and Myod1 in fibroblasts, with both transcription factors accessing both neuronal and myogenic targets. We also observed similar changes in chromatin accessibility and transcriptional activation. Yet, Myod1 predominantly induced a muscle program and Ascl1 a neuronal program. We found that differences in binding affinity at key targets resulted in largely distinct reprogramming outcomes. Accordingly, exchanging Myod1’s C-terminal protein-protein interacting domain and DNA-binding basic domain with those of Ascl1 induces an Ascl1-like binding and converts Myod1 into a pro-neuronal factor. Finally, we found that co-expression of Myod1 with the transcriptional repressor Myt1l inhibits induction of the muscle program and yields functional neuronal cells. Our findings are compatible with the notion that pioneer factor activity is associated with high-affinity protein-DNA and suggest that promiscuous binding of pioneer factors can induce unspecific lineage features which need to be kept in check by co-factor interactions.

2020-02-03 | GSE126413 | GEO

Pro-neuronal activity of Myod1 due to promiscuous binding [RNA-seq]

2020-02-03 | GSE126412 | GEO

Pro-neuronal activity of Myod1 due to promiscuous binding [H3K27ac ChIP-seq]

2020-02-03 | GSE126411 | GEO