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Transgenic expression of human PON1 in Drosophila melanogaster under the Tub driver

ABSTRACT: Human paraoxonase-1 (PON1) is an enzyme with lactonase, esterase and phosphotriesterase activity that has been associated with multiple phenotypes. We expressed hPON1 ubiquitously in Drosophila melanogaster using the Gal4 system Tub driver.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE29534 | GEO | 2011/05/26

SECONDARY ACCESSION(S): PRJNA141373

REPOSITORIES: GEO

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Transgenic expression of human PON1 in Drosophila melanogaster under the Tub driver

Project description:Human paraoxonase-1 (PON1) is an enzyme with lactonase, esterase and phosphotriesterase activity that has been associated with multiple phenotypes. We expressed hPON1 ubiquitously in Drosophila melanogaster using the Gal4 system Tub driver. RNA from 30 flies from each of 3 genotypes was used to hybridize 4 arrays in a 12-plex array (4 technical replicates, 3 genotypes).

2011-05-26 | E-GEOD-29534 | biostudies-arrayexpress

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Repeated gestational exposure of mice to chlorpyrifos oxon is associated with paraoxonase 1 (PON1)-modulated effects in maternal and fetal tissues

Project description:Chlorpyrifos oxon (CPO), the toxic metabolite of the organophosphorus (OP) insecticide chlorpyrifos, causes developmental neurotoxicity in humans and rodents. CPO is hydrolyzed by paraoxonase-1 (PON1), with protection determined by PON1 levels and the human Q192R polymorphism. To examine how the Q192R polymorphism influences fetal toxicity associated with gestational CPO exposure, we measured biomarker inhibition and fetal-brain gene expression in wild-type (PON1+/+), PON1-knockout (PON1-/-), and tgHuPON1R192 and tgHuPON1Q192 transgenic mice. Pregnant mice exposed dermally to 0, 0.50, 0.75 or 0.85 mg/kg/d CPO from gestational days (GD) 6 through 17 were sacrificed on GD18. Biomarkers of CPO exposure inhibited in maternal tissues included brain acetylcholinesterase (AChE), RBC acylpeptide hydrolase (APH), plasma butyrylcholinesterase (BChE) and carboxylesterase (CES). Fetal plasma BChE was inhibited in PON1-/- and tgHuPON1Q192, but not PON1+/+ or tgHuPON1R192 mice. Fetal brain AChE and plasma CES were inhibited in PON1-/- mice, but not in other genotypes.

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Cerebellum from mice exposed to chronic low-level chlorpyrifos oxon

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2011-11-09 | E-GEOD-25250 | biostudies-arrayexpress

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