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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [sc_islet]

ABSTRACT: The goal of this study was to optimize single-cell long-read RNA sequencing in pancreatic islets to enable accurate isoform-level gene expression analysis. We aimed to overcome key technical limitations—including insufficient read lengths, high error rates, and transcript abundance bias—that have hindered long-read sequencing in single cells. Specifically, we compared 5’ and 3’ library preparation strategies and evaluated transcript depletion methods to improve detection of low-abundance isoforms. Our objective was to establish a robust protocol that enhances isoform identification and reveals differential transcript usage across pancreatic islet cell types.

ORGANISM(S): Mus musculus

PROVIDER: GSE295353 | GEO | 2026/01/26

REPOSITORIES: GEO

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Dataset's files

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			Action	DRS
		Other
	GSE295353_MSH_1_mod_5prime_pass.gene_expression.counts.subset.tsv.gz	Tabular
	GSE295353_MSH_1_mod_5prime_pass.transcript_expression.counts.subset.tsv.gz	Tabular
	GSE295353_islet_5p_mod.gene_expression.counts.subset.tsv.gz	Tabular
	GSE295353_islet_5p_mod.transcript_expression.counts.subset.tsv.gz	Tabular

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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [sc_spleen]

Project description:The goal of this study was to optimize single-cell long-read RNA sequencing in pancreatic islets to enable accurate isoform-level gene expression analysis. We aimed to overcome key technical limitations—including insufficient read lengths, high error rates, and transcript abundance bias—that have hindered long-read sequencing in single cells. Specifically, we compared 5’ and 3’ library preparation strategies and evaluated transcript depletion methods to improve detection of low-abundance isoforms. Our objective was to establish a robust protocol that enhances isoform identification and reveals differential transcript usage across pancreatic islet cell types.

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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [bulk_islet]

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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [bulk_islet]

Project description:Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [bulk_islet]

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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [sc_spleen]

Project description:Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [sc_spleen]

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Optimizing Single-Cell Long-Read Sequencing for Enhanced Isoform Detection in Pancreatic Islets [sc_islet]

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