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Taurine improves bovine oocytes maturation through recovering mitochondria dysfunction and oxidative stress-induced apoptosis after Microplastics exposure

ABSTRACT: Plastic is widespread in our lives, releasing various Microplastics (MPs) with toxicity. In recent years, the potential threat of MPs on reproductive system arouses public concern. Numerous reports have focused on its damage to spermatogenesis. Nevertheless, the toxicity of MPs on female reproduction is unclear. Here, we explored this question using bovine oocyte. We found that MPs disrupts spindle organization, chromosomes alignment and actin assembly, leading to failed maturation of bovine oocytes. Meanwhile, cortical granule secretion is obstructed, suggesting the failure of cytoplasmic maturation. Therefore, embryonic development is impacted. In transcriptome results, MPs induces the expression change of Mitochondrial-related genes, reflecting the damage of MPs are mediated by mitochondrial functions. MPs indeed causes oxidative stress, DNA damage and apoptosis. Taurine is capable for stabilizing cellular antioxidant level. We surprisingly find that Taurine suppresses mitochondrial dysfunction, reversing the failure of oocyte maturation and embryo development after MPs exposure. Collectively, we reveal the reproduction toxicity of MPs on bovine oocytes, and demonstrates the restorative effect of Taurine against MPs.

ORGANISM(S): Bos taurus

PROVIDER: GSE295716 | GEO | 2025/08/07

REPOSITORIES: GEO

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Project description:In mammals, the capacity of the female germ cell, the oocyte, to develop into embryo is acquired throughout meiotic maturation. Immature oocyte cannot be fertilized while mature oocyte is apt to accept spermatozoa and to develop an embryo. In a follicle, the oocyte is surrounded by mural granulosa cells (GC) and is physically and metabolically coupled with specialized granulosa cumulus cells (CC) which play an important role in oocyte maturation and fertilization. Factors expressing in GC and CC during maturation may reflect the oocyte quality, i.e. its capacity to be fertilized and assure early embryo development. However, the modifications of the content and the amount of peptide/proteins in the oocyte and the surrounding CC during oocyte maturation are mostly unknown and so there is not an accurate way of evaluating/monitoring how different in vitro maturation (IVM) protocols being in use in assisted reproduction technologies, can affect the process. In this context, Intact Cell MALDI-TOF Mass Spectrometry (ICM-MS) was applied to bovine follicular cells (bovine single oocytes, cumulus cells and granulosa cells) in order to characterize proteomic changes that occur in the follicle during female gamete development. In order to characterize finely endogenous molecular species observed on ICM-MS profiles and to identify markers of interest with their post-translational modifications, we carried out top-down proteomic on the different follicular cells from oocyte, oocyte-cumulus complexes, cumulus cells and granulosa cells protein extracts. Prior to top-down MS using a dual linear ion trap Fourier Transform Mass Spectrometer LTQ Orbitrap Velos, depending on the amount of available biological material, we employed three analytic strategies as a direct infusion, a mono-dimensional liquid chromatography (µLC-1D-MS/MS) and an off-line multi-dimensional liquid chromatography combining four fractionations (based on reverse phase or gel filtration LC) to µLC-MS/MS. Here, we deposited our dataset from µLC-1D-MS/MS (analyses of oocytes, oocyte-cumulus complexes, cumulus cells and granulosa cells protein extracts) and MDLC-MS/MS (analyses of granulosa cells protein extract).

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Taurine improves bovine oocytes maturation through recovering mitochondria dysfunction and oxidative stress-induced apoptosis after Microplastics exposure

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