Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptomics of Pseudomonas aeruginosa PA14 upon deletion of the sigma factor RpoS

ABSTRACT: We report RNA-seq data of Pseudomonas aeruginosa PA14 (UCBPP-PA14) and an isogenic rpoS-STOP mutant generated using CRISPR/Cas9 base editing. Transcriptomic profiling during exponential growth in rich medium highlights the regulatory influence of RpoS. These data enable exploration of RpoS-dependent responses in the virulent PA14 strain.

ORGANISM(S): Pseudomonas aeruginosa

PROVIDER: GSE295781 | GEO | 2025/04/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptomics of Pseudomonas aeruginosa PA14 upon deletion of the sigma factor RpoS

Project description:Transcriptomics of Pseudomonas aeruginosa PA14 upon deletion of the sigma factor RpoS

| PRJNA1256354 | ENA

Pseudomonas aeruginosa PA14

Project description:PA14-PA2444 vs. PA14-WT biofilm cells

| PRJNA140729 | ENA

Pseudomonas aeruginosa PA14 isolate:PA14

Project description:Pseudomonas aeruginosa PA14 isolate:PA14 Genome sequencing

| PRJNA1028549 | ENA

Expression profile for higA mutant of Pseudomonas aeruginosa PA14

Project description:The previously uncharacterized proteins HigB (unannotated) and HigA (PA4674) of Pseudomonas aeruginosa PA14 were found to form a type II TA system in which antitoxin HigA masks the RNase activity of toxin HigB through direct binding. To determine the physiological role of HigB/HigA in P. aeruginosa, a whole-transcriptome experiment was performed for the higA antitoxin deletion mutant of the PA14 strain compared to the wild-type PA14 strain. The rationale was that for the strain that lacks the antitoxin, the effect of the toxin could be discerned due to enhanced activity of the toxin. Furthermore, toxin HigB reduces production of the virulence factors pyochelin, pyocyanin, swarming, and biofilm formation.

2016-11-04 | GSE74730 | GEO

Pseudomonas aeruginosa PA14

Project description:Pseudomonas aeruginosa PA14

| PRJNA644758 | ENA

Proteomics of Pseudomonas aeruginosa PAO1 and PA14

Project description:Recent studies have shown that the concentrations of proteins expressed from orthologous genes are often conserved across organisms, and to a greater extent than the abundances of the corresponding mRNAs. However, such studies have not distinguished between evolutionary (e.g., sequence divergence) and environmental (e.g., growth condition) effects on the regulation of steady-state protein and mRNA abundances. Here we systematically investigated the transcriptome and proteome of two closely related Pseudomonas aeruginosa strains, PAO1 and PA14, under identical experimental conditions, thus controlling for environmental effects. For 703 genes observed by both shotgun proteomics and microarray experiments, we find that the protein-to-mRNA ratios are highly correlated between orthologous genes in the two strains, to an extent comparable to protein and mRNA abundances. In spite of this high molecular similarity between PAO1 and PA14, we found that several metabolic, virulence, and antibiotic resistance genes are differentially expressed between the two strains, mostly at the protein but not at the mRNA level. Our data demonstrate that post-transcriptional regulation is important for understanding the discordance between mRNA and protein abundance.

2014-05-08 | PXD000684 | Pride

Genomic DNA hybridization of Pseudomonas aeruginosa strains PAO1 and PA14

Project description:Genomic DNA from Pseudomonas aeruginosa strains PAO1 and PA14

2015-11-15 | GSE67038 | GEO

Pseudomonas aeruginosa PA14

Project description:Pseudomonas aeruginosa PA14 Transcriptome

| PRJNA421184 | ENA

Pseudomonas aeruginosa PA14

Project description:Experimental evolution of uninfected PA14 and PA14 infected with temperate virus DMS3

| PRJNA1021667 | ENA

Pseudomonas aeruginosa PA14

Project description:Pseudomonas aeruginosa PA14 Genome sequencing