Single cell analysis of naïve or AAV transduced NSG-PiZ mouse livers

ABSTRACT: To determine whether different hepatotropic AAV capsids can mediate transduction of human hepatocytes in liver-humanized chimeric mice we performed an experiment in NSG-PiZ mice. NSG-PiZ mice were simultaneously administered barcoded AAV vectors with capsids derived from AAV8, AAV9, AAVrh10, AAVDJ/8 and AAV.LK03 at a total dose of 1E12 vg per mouse. At 14 days post delivery naïve and AAV treated mice were sacrificed and livers perfused to generate single cell suspensions for scRNA-seq. We transplanted mice with human hepatocytes from 2 donors (4 mice per donor) and half of the mice from each donor were adminsitered AAV.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE295881 | GEO | 2025/04/29

REPOSITORIES: GEO