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Comparison of gene expression profiles of effector T cells from leptin-receptor-deficient mice and wild type mice

ABSTRACT: We have employed whole genome microarray expression profiling as a discovery platform to identify genes differentially expressed during leptin receptor deficiency, which is partly responsible for the inhibition of T cell effector functions. Mouse CD4+CD25- T cells were purified from leptin-receptor-deficient mice and wild type mice, and stimulated with anti-CD3 and anti-CD28 for 12h. Six mice of each genotype were used.

ORGANISM(S): Mus musculus

PROVIDER: GSE29607 | GEO | 2012/09/05

SECONDARY ACCESSION(S): PRJNA153819

REPOSITORIES: GEO

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Comparison of gene expression profiles of effector T cells from leptin-receptor-deficient mice and wild type mice

Project description:We have employed whole genome microarray expression profiling as a discovery platform to identify genes differentially expressed during leptin receptor deficiency, which is partly responsible for the inhibition of T cell effector functions. Mouse CD4+CD25- T cells were purified from leptin-receptor-deficient mice and wild type mice, and stimulated with anti-CD3 and anti-CD28 for 12h. Six mice of each genotype were used. Leptin receptor deficiency-induced gene expression profiles were evaluated in mouse effector T cells from db/db mice and compared to the ones observed in their WT counterpart (db/+ mice). Two independent experiments were performed, and in each of them, 3 mice/group were pooled.

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Expression data from livers of F2 mice (B57BL/6 X DBA/2) deficient in leptin receptor (db/db)

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