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M. smegmatis vapBC::aphA-3 ΔvapBC mutant (JR121) harboring tetracycline inducible vector pMind containing the vapBC operon (pJR230) and the pMind vector containing the vapC toxin gene (pJR29)

ABSTRACT: Transcriptional profiling of M. smegmatis JR121 expressing VapC and VapBC grown in flasks on Hartmans de Bont medium supplemented with 0.2% glycerol

ORGANISM(S): Mycolicibacterium smegmatis MC2 155 Mycolicibacterium smegmatis

PROVIDER: GSE29631 | GEO | 2012/06/15

SECONDARY ACCESSION(S): PRJNA141261

REPOSITORIES: GEO

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M. smegmatis vapBC::aphA-3 M-NM-^TvapBC mutant (JR121) harboring tetracycline inducible vector pMind containing the vapBC operon (pJR230) and the pMind vector containing the vapC toxin gene (pJR29)

Project description:Transcriptional profiling of M. smegmatis JR121 expressing VapC and VapBC grown in flasks on Hartmans de Bont medium supplemented with 0.2% glycerol Comparing transcriptional response of strain JR121 to conditional expression of VapC toxin compared with the expression of VapBC complex. Biological replicates: 4 independently grown and harvested. One replicate per array.

2012-06-14 | E-GEOD-29631 | biostudies-arrayexpress

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2014-10-31 | E-GEOD-56057 | biostudies-arrayexpress

M. smegmatis pruR::Tn611 mutant (MB100pruR)

Project description:Transcriptional profiling of M. smegmatis MB100pruR vs. MB100 (isogenic parent) grown in serumvials on Hartmans de Bont medium supplemented with glycerol and proline

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Bedaquiline-induced transcriptional response of Mycobacterium smegmatis mc2 155

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The AmtR regulon of Mycobacterium smegmatis - Transcription profile of an M. smegmatis amtR mutant (JR258) compared to M. smegmatis mc2155 (wild-type)

Project description:To identify the AmtR regulon of Mycobacterium smegmatis, we created a markerless deletion of the amtR gene in the background of strain M. smegmatis mc2155 (wild-type) and compared the transcription profile of both strains grown in batch culture under aerobic conditions on Hartmans de Bont medium supplemented with glycerol (carbon source) and lysine (sole nitrogen source) using microarray. Cells were harvested in early exponential growth stage.

2016-08-31 | E-MTAB-4857 | biostudies-arrayexpress

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RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r)

Project description:RNA-seq of Mycobacteriophage Island3 infection of Mycolicibacterium smegmatis mc2155, Mycolicibacterium smegmatis mc2155(Butters), and Mycolicibacterium smegmatis mc2155(Buttersgp57r) to assess the impact of Butters lysogen and specifically Buttersgp57r on transcript levels of island3 during infection.

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Mycobacterium smegmatis: I-SceI homing endonuclease generated genomic DNA damage in Darr strains expressing ArrWT, ArrH18A, ArrD83A, or an empty vector vs WT M. smegmatis Mc2155 (mgm182, site(+))

Project description:Transcriptional profiling of Mycobacterium smegmatis comparing strains undergoing I-SceI generated DNA damage at a single genomic locus Gene designations are the updated annotation Four comparisons were made, all with log phase cultures and no ATc added. Darr Site(+) with an empty vector compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing WT Arr compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing ArrH18A compared to wild-type M. smegmatis Site(+), Darr Site(+) with a vector expressing Arr D83A compared to wild-type M. smegmatis Site(+). 3 biological replicates of each strain for each experiment.

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