Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Total RNA-seq of mouse olfactory epithelium in response to single odorants and binary mixtures

ABSTRACT: Total RNA was extracted from olfactory epithelium tissues of mice exposed to single odorants (acetophenone, decanal, octanal, and cis-3-hexenol) or binary mixtures (acetophenone–decanal and octanal–cis-3-hexenol). The extracted RNA was subjected to high-throughput sequencing to profile genome-wide transcriptional changes across all cell types of the olfactory epithelium following odor stimulation. This dataset provides a baseline reference for comparing odor-induced gene expression programs in the olfactory epithelium with transcriptomic profiles obtained from odor-activated neuronal populations enriched by pS6 immunoprecipitation.

ORGANISM(S): Mus musculus

PROVIDER: GSE296359 | GEO | 2025/10/09

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

pS6 immunoprecipitation RNA-seq of mouse olfactory epithelium in response to single odorants, binary mixtures, and complex fragrances

Project description:Phosphorylation of ribosomal protein S6 (pS6) serves as a molecular marker of neuronal activation by external stimuli. In this study, olfactory epithelium tissues were collected from mice exposed to single odorants (acetophenone, decanal, octanal, and cis-3-hexenol), binary mixtures (acetophenone–decanal and octanal–cis-3-hexenol), or complex fragrances (floral, mint, and floral–mint combination). To selectively capture transcripts undergoing active translation in odor-activated cells, pS6-associated ribosome complexes were isolated from tissue lysates through immunoprecipitation. The enriched mRNAs were subsequently purified and subjected to RNA sequencing, enabling transcriptomic profiling specifically within odor-activated neuronal populations, particularly olfactory sensory neurons. This approach provides a targeted view of gene expression programs induced by diverse odor stimulation paradigms in the heterogeneous olfactory epithelium.

2025-10-09 | GSE296360 | GEO

pS6-IP RNA-seq of mouse olfactory epithelium upon binary odor mixture stimulation under different delivery methods

Project description:Phosphorylation of ribosomal protein S6 (pS6) serves as a molecular marker of neuronal activation by external stimuli. In this study, olfactory epithelium tissues were obtained from mice exposed to binary odor mixtures consisting of acetophenone–decanal or octanal–cis-3-hexenol. To examine how different delivery methods of odor presentation influence neural responses, two paradigms for binary mixtures were employed. In the “+” condition, the two odorants were directly combined on a single piece of filter paper, allowing interaction prior to volatilization. In contrast, in the “&” condition, the odorants were applied separately to two filter papers placed in close proximity (~1 mm apart), such that the components mixed only in the air upon evaporation. Using these approaches, four experimental odor groups were generated (A+D, A&D, O+C, and O&C), along with a no-odor control, resulting in a total of five groups. Following stimulation, pS6-associated ribosome complexes were isolated from olfactory epithelium lysates through immunoprecipitation, selectively enriching mRNAs undergoing active translation in odor-activated cells. RNA sequencing of these samples enabled transcriptomic profiling within activated neuronal populations, providing insights into gene expression programs associated with distinct delivery methods of binary odor stimulation.

2025-10-09 | GSE306619 | GEO

Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

Project description:We present a high-throughput in vivo method to identify odorant receptors responding to odorants, using phosphorylated ribosome immunoprecipitation of mRNA from olfactory epithelium of odor-stimulated mice followed by RNA-Seq. pS6-IP RNA-Seq in odor stimulated vs control mice olfactory epithelium

2015-08-24 | E-GEOD-59324 | biostudies-arrayexpress

Molecular profiling of activated olfactory neurons identifies odorant receptors for odors in vivo

2015-08-24 | GSE59324 | GEO

pS6 immunoprecipitation RNA-seq of mouse olfactory epithelium in response to single odorants, binary mixtures, and complex fragrances

Project description:pS6 immunoprecipitation RNA-seq of mouse olfactory epithelium in response to single odorants, binary mixtures, and complex fragrances

| PRJNA1258983 | ENA

CD20 is a mammalian odorant receptor expressed in a subset of olfactory sensory neurons that mediates innate avoidance of predators

Project description:The mammalian olfactory system detects and discriminates between millions of odorants to elicit appropriate behavioral responses. While much has been learned about how olfactory sensory neurons detect odorants and signal their presence, how specific innate, unlearned behaviors are initiated in response to ethologically relevant odors remains poorly understood. Here, we show that the 4-transmembrane protein CD20, also known as MS4A1, is expressed in a previously uncharacterized subpopulation of olfactory sensory neurons in the main olfactory epithelium of the murine nasal cavity and functions as a mammalian odorant receptor that recognizes compounds produced by mouse predators. While wildtype mice avoid these predator odorants, mice genetically deleted of CD20 do not appropriately respond. Together, this work reveals a novel CD20-mediated odor-sensing mechanism in the mammalian olfactory system that triggers innate behaviors critical for organismal survival.

2023-08-08 | GSE240378 | GEO

Total RNA-seq of mouse olfactory epithelium in response to single odorants and binary mixtures

Project description:Total RNA-seq of mouse olfactory epithelium in response to single odorants and binary mixtures

| PRJNA1258984 | ENA

pS6-IP RNA-seq of mouse olfactory epithelium upon binary odor mixture stimulation under different delivery methods

Project description:pS6-IP RNA-seq of mouse olfactory epithelium upon binary odor mixture stimulation under different delivery methods

| PRJNA1311487 | ENA

Rapid transcriptional response to physiological neuronal activity in vivo revealed by transcriptome sequencing

Project description:mRNA-seq of olfactory bulbs from odor-deprived and odor-stimulated mice (RNA-seq done in triplicates) Control mice were kept in a clean air environment (using an activated carbon filter). Stimulated mice were exposed to a cocktail of odorants for 30 minutes ("30 min" time point), or for 30 minutes followed by a period of 90 minutes of clean air ("120 min" time point.) Mice were sacrificed immediately at end of time point, and olfactory bulbs were dissected out for RNA extraction and RNA-FISH. Three adult mice (6-8 weeks of age or older) were used in each condition.

2014-06-27 | E-GEOD-58843 | biostudies-arrayexpress

Cystic fibrosis causes olfactory system defects by altering progenitor cell proliferation (scRNA-Seq)

Project description:A reduced sense of smell has been reported in people with cystic fibrosis (CF). These olfactory defects have largely been attributed to secondary manifestations of the disease, such as inflammation of the nasal mucosa. Here we show that CFTR, the gene responsible for CF, is expressed in proliferating olfactory human cells and that newborn CFTR null pigs display ultrastructural abnormalities in the olfactory epithelium and olfactory bulbs. In the absence of CFTR, olfactory sensory neurons still produce odor-evoked activity, but mutant animals display defective odor-guided suckling behavior after birth. Consistent with epithelial changes, we found a reduced expression of genes implicated in cell cycle and development in globose basal cells (GBCs), the neurogenic progenitor cells in the olfactory epithelium. Targeted sequencing revealed enhanced CFTR expression in the subpopulation of GBCs that is actively proliferating. Furthermore, CFTR loss caused a global reduction in the number of sensory neurons and altered olfactory receptors expression. Our findings highlight a previously unknown role of CFTR in olfactory system function by regulating progenitor cell proliferation in the olfactory epithelium.

2022-12-31 | GSE197189 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data