Poly(A)-RNA-Seq sequencing of Aspergillus fumigatus strain CEA10 at 37°C and 42°C
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ABSTRACT: Experimantal validation for the 5' and 3' ends of poly(A)-RNA in Aspergillus fumigatus is currently lacking. The dataset here was used a total RNA-Seq control for poly(A)-enriched RNAs of Aspergillus fumigatus in comparison with 5' and 3' End-Seq data.
Project description:Experimantal validation for the 3' end of poly(A)-RNA in Aspergillus fumigatus is currently lacking. Therefore we performed 3'RNA-End sequencing from 24-h mycelium grown in AMM at 37°C and 42°C with a library kit purchased from Eclipse Bioinnovations.
Project description:Experimantal validation for the 5' end of poly(A)-RNA in Aspergillus fumigatus is currently lacking. Therefore we performed 5'RNA-End sequencing from 24-h mycelium grown in AMM at 37°C and 42°C with a library prepration kit purchased from Eclipse Bioinnovations.
Project description:Genomic DNA from five strains, Aspergillus fumigatus Af71, Aspergillus fumigatus Af294, Aspergillus clavatus, Neosartorya fenneliae, and Neosartorya fischeri, were co-hybridized with that of Aspergillus fumigatus Af293 and compared.
Project description:Amphotericin B (AMB) is the most widely used polyene antifungal drug for the treatment of systemic fungal infections including invasive aspergillosis. We aimed to understand molecular targets of AMB in Aspergillus fumigatus (Afu) by genomic approaches. Keywords: Aspergillus fumigatus treated with amphotericin B for 24 hours