Single-cell spatial transcriptomic profiling of cultured cells and engineered tissues without embedding or sectioning
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ABSTRACT: The deposited data corresponds to the development of a protocol for spatial transcriptomic profiling of 2D engineered tissues and cell cultures that are not compatible with standard embedding and sectioning. By fixing and permeabilizing cells grown directly on a microscope glass slide, we demonstrate how Visium HD spatial gene expression kit can be used to investigate the function of these cells. Manuscript link: pending Github repository: https://github.com/HaylieHelms/vhd-scbioprint1
ORGANISM(S): Homo sapiens
PROVIDER: GSE296623 | GEO | 2025/07/09
REPOSITORIES: GEO
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