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Expression data from rat skeletal muscle at 0 and 48 hours of postmortem interval

ABSTRACT: Accurate estimation of the postmortem interval is critical in forensic investigations to determine the time since death. While traditional methods offer limited precision, molecular approaches based on gene expression have shown promise. mRNA retains detectable and quantifiable expression changes that reflect biological processes occurring after death. In this study, we analyzed the transcriptome of rat skeletal muscle at 0 hours (control) and 48 hours of post mortem interval to identify differentially expressed mRNAs that may serve as potential biomarkers, aiming to enhance the accuracy and reliability of PMI estimation in forensic science. Microarray analysis using the Affymetrix Clariom S Rat array (Affymetrix) facilitated a comprehensive overview of the skeletal muscle transcriptome at 48 hours of postmortem interval. This platform allowed for the simultaneous quantification of thousands of transcripts, revealing widespread gene expression alterations associated with the postmortem interval. The resulting transcriptomic profile not only provided insight into molecular changes occurring after death but also highlighted specific mRNAs as potential biomarker candidates for PMI estimation in forensic contexts.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE297166 | GEO | 2025/12/02

REPOSITORIES: GEO

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