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An improved nuclei isolation protocol from leaf tissue for single-cell transcriptomics

ABSTRACT: We developed an alternative method for isolating nuclei to be utilized in scRNA-seq experiments from Zea mays leaves with reduced chloroplast contamination. By effectively removing chloroplasts during the FACS step of our protocol, using the autofluorescence from the chloroplasts, we achieved improved alignment of reads to the genome and transcriptome. Our enhanced protocol offers a valuable solution for applying snRNA-seq in tissues with a high content of chloroplasts.

ORGANISM(S): Zea mays

PROVIDER: GSE297213 | GEO | 2025/07/17

REPOSITORIES: GEO

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