Dataset Information
Transcriptional activity during seed imbibition is conserved, robust and required for germination.
Ontology highlight
ABSTRACT:
ORGANISM(S): Arabidopsis thaliana Brassica napus
PROVIDER: GSE297302 | GEO | 2026/07/24
REPOSITORIES: GEO
Similar Datasets
Project description:Many plant lncRNAs regulate gene expression by binding to chromatin, but how they are retained at the target loci is unclear. We identify a new, chromatin-localized lncRNA - MUSHER, which activates two parallel regulatory pathways to increase Arabidopsis seed dormancy. MUSHER is upregulated in response to high temperatures, contributing to the induction of secondary dormancy. It promotes DOG1 expression by recruitment of the CPSF complex to enhance the proximal cleavage and polyadenylation at the DOG1 transcript. It also increases ABA sensitivity in seeds by activating PIR1 gene transcription. These genes, located on different chromosomes, are both bound by MUSHER, despite lacking sequence homology. The chromatin association of MUSHER enables the integration of the DOG1- and ABA pathways to adjust seed germination timing. Additionally, MUSHER and other lncRNAs interact with U1 snRNP, which is required for their chromatin localisation, revealing a novel function of U1 snRNP in plants. Funded by the National Science Centre Grant, Poland (UMO-2021/41/B/NZ3/02605, UMO‐2024/53/B/NZ3/02252, UMO‐2024/53/B/NZ1/03741).
2025-07-14 | GSE274757 | GEO
Project description:The DXO family of proteins participates in eukaryotic mRNA 5'-end quality control, removal of non-canonical NAD+ cap and maturation of fungal rRNA precursors. In this work, we characterize DXO1, the Arabidopsis thaliana DXO homolog. We demonstrate that the plant-specific modification within the active site negatively affects 5'-end capping surveillance properties of DXO1, but has only a minor impact on its strong deNADding activity. Unexpectedly, catalytic activity does not contribute to striking morphological and molecular aberrations observed upon DXO1 knockout in plants, which include growth and pigmentation deficiency, global transcriptomic changes and accumulation of RNA quality control siRNAs. Conversely, these phenotypes depend on the plant-specific N-terminal extension of DXO1. Pale-green coloration of DXO1-deficient plants and our RNA-seq data reveal that DXO1 affects chloroplast-localized processes. We propose that DXO1 mediates the connection between RNA turnover and retrograde chloroplast-to-nucleus signaling independently of its deNADding properties. Founded by National Science Centre Grant, Poland (UMO-2013/08/M/NZ1/00931, UMO-2018/29/B/NZ3/01980, UMO‐2021/40/Q/NZ1/00014, UMO‐2014/13/B/NZ3/00405, MINIATURA 2017/01/X/NZ1/00332, UMO‐2021/41/B/NZ3/02605).
2019-02-08 | GSE99600 | GEO
Project description:Arabidopsis thaliana contains two nuclear XRN2/3 5'-3' exonucleases that are homologs of yeast and human Rat1/Xrn2 proteins involved in the processing and degradation of several classes of nuclear RNAs and in transcription termination of RNA polymerase II. Using strand-specific short read sequencing we show that knockdown of XRN3 leads to an altered expression of hundreds of genes and the accumulation of uncapped and polyadenylated read-through transcripts generated by inefficiently terminated Pol II. Our data support the notion that XRN3-mediated changes in the expression of a subset of genes are caused by upstream read-through transcription and these effects are enhanced by RNA-mRNA chimeras generated in xrn3 plants. In turn, read-through transcripts that are antisense to downstream genes may trigger production of siRNA. Our results highlight the importance of XRN3 exoribonuclease in Pol II transcription termination in plants and show that disturbance in this process may significantly alter gene expression. Founded by National Science Centre Grant, Poland (UMO-2018/29/B/NZ3/01980, UMO‐2021/40/Q/NZ1/00014, UMO‐2014/13/B/NZ3/00405, MINIATURA 2017/01/X/NZ1/00332, UMO‐2021/41/B/NZ3/02605).
2018-01-24 | GSE95473 | GEO
Project description:Decapping 5'-3' exoribonucleases from the DXO/Rai1 family are highly conserved among eukaryotes and exhibit diverse enzymatic activities depending on the organism. The biochemical and structural properties of the plant DXO1 differ from the yeast and animal counterparts, which is reflected in the in vivo functions of this enzyme. Here we show that Arabidopsis DXO1 contributes to the efficient processing of rRNA precursors in both nucleolar/cytosolic and chloroplast maturation pathways. However, the processing defects in DXO1-deficient plants do not depend on the catalytic activity of the enzyme but rely on its plant-specific N-terminal extension, which is responsible for the interaction with the mRNA cap methyltransferase RNMT1. Our RNA sequencing analyses show that the dxo1 mutation deregulates the expression of many ribosomal protein genes, most likely leading to inefficient or delayed pre-rRNA maturation. These phenotypes are partially suppressed by RNMT1 overexpression, suggesting that defective cap synthesis may be responsible, at least to some extent, for the observed effects. Founded by National Science Centre Grant, Poland (UMO‐2018/29/B/NZ3/01980, UMO‐2021/40/Q/NZ1/00014, UMO‐2014/13/B/NZ3/00405, MINIATURA 2017/01/X/NZ1/00332, UMO‐2021/41/B/NZ3/02605).
2025-02-11 | GSE210631 | GEO
Project description:Decapping 5'-3' exoribonucleases from the DXO/Rai1 family are highly conserved among eukaryotes and exhibit diverse enzymatic activities depending on the organism. The biochemical and structural properties of the plant DXO1 differ from the yeast and animal counterparts, which is reflected in the in vivo functions of this enzyme. Here we show that Arabidopsis DXO1 contributes to the efficient processing of rRNA precursors in both nucleolar/cytosolic and chloroplast maturation pathways. However, the processing defects in DXO1-deficient plants do not depend on the catalytic activity of the enzyme but rely on its plant-specific N-terminal extension, which is responsible for the interaction with the mRNA cap methyltransferase RNMT1. Our RNA sequencing analyses show that the dxo1 mutation deregulates the expression of many ribosomal protein genes, most likely leading to inefficient or delayed pre-rRNA maturation. These phenotypes are partially suppressed by RNMT1 overexpression, suggesting that defective cap synthesis may be responsible, at least to some extent, for the observed effects. Founded by National Science Centre Grant, Poland (UMO‐2018/29/B/NZ3/01980, UMO‐2021/40/Q/NZ1/00014, UMO‐2014/13/B/NZ3/00405, MINIATURA 2017/01/X/NZ1/00332, UMO‐2021/41/B/NZ3/02605).
2025-02-11 | GSE210614 | GEO
Project description:Multiple factors control primary seed dormancy established during seed maturation and secondary seed dormancy established when a non-dormant seed is exposed to adverse conditions during imbibition. A key player in the control of both primary and secondary dormancy in Arabidopsis thaliana is the DOG1 gene, whose expression is extensively regulated at the transcriptional level. Despite its importance, the influence of posttranscriptional RNA processing and mRNA storage of DOG1 on the determination of dormancy depth remains elusive. We show that the UBA2A protein, a member of the hnRNP family, suppresses primary and secondary seed dormancy through the regulation of the DOG1 gene expres-sion at the posttranscriptional level. Surprisingly, single-molecule FISH, chromatin-attached RNA analysis and Pol II ChIP demonstrated that UBA2A do not control DOG1 gene transcription but rather DOG1 mRNA chromatin retention. Our study highlights chromatin retention as an important step in DOG1 gene expression regulation during dormancy establishment and shows that UBA2A like its human homolog hnRNPAB is implicated in RNA transport in the cell. Funded by National Science Centre Grant, Poland UMO-2021/41/B/NZ3/02605.
2025-10-15 | GSE245457 | GEO
Project description:Eukaryotic genomes are pervasively transcribed by RNA polymerase II. Yet, the molecular and biological implications of such a phenomenon are still largely puzzling. Here, we describe noncoding RNA transcription upstream of the Arabidopsis thaliana DOG1 gene, which governs salt stress responses and is a key regulator of seed dormancy. We find that expression of the DOG1 gene is induced by salt stress, thereby causing a delay in seed germination. We uncover extensive transcriptional activity on the promoter of the DOG1 gene, which produces a variety of lncRNAs. These lncRNAs, named PUPPIES, are co-directionally transcribed and extend into the DOG1 coding region. We show that PUPPIES RNAs respond to salt stress and boost DOG1 expression, resulting in delayed germination. This positive role of pervasive PUPPIES transcription on DOG1 gene expression is associated with augmented pausing of RNA polymerase II, slower transcription and higher transcriptional burst size. These findings highlight the positive role of upstream co-directional transcription in controlling transcriptional dynamics of downstream genes. Funded by the National Science Centre Grant, Poland UMO-2021/41/B/NZ3/02605.
2023-02-01 | GSE208755 | GEO
Project description:NOL12 5'-3' exoribonucleases, conserved among eukaryotes, play important roles in pre-rRNA processing, ribosome assembly and export. The most well-described yeast counterpart, Rrp17, is required for maturation of 5.8 and 25S rRNAs, whereas human hNOL12 is crucial for the separation of the large (LSU) and small (SSU) ribosome subunit rRNA precursors. In this study we demonstrate that plant AtNOL12 is also involved in rRNA biogenesis, specifically in the processing of the LSU rRNA precursor, 27S pre-rRNA. Importantly, the absence of AtNOL12 alters the expression of many ribosomal protein and ribosome biogenesis genes. These changes could potentially exacerbate rRNA biogenesis defects, or, conversely, they might stem from the disturbed ribosome assembly caused by delayed pre-rRNA processing. Moreover, exposure of the nol12 mutant to stress factors, including heat and pathogen Pseudomonas syringae, enhances the observed molecular phenotypes, linking pre-rRNA processing to stress response pathways. The aberrant rRNA processing, dependent on AtNOL12, could impact ribosome function, as suggested by improved mutant resistance to ribosome-targeting antibiotics. Despite extensive studies, the pre-rRNA processing pathway in plants remains insufficiently characterized. Our investigation reveals the involvement of AtNOL12 in the maturation of rRNA precursors, correlating this process to stress response in Arabidopsis. These findings contribute to a more comprehensive understanding of plant ribosome biogenesis. Founded by National Science Centre Grant, Poland (UMO-2018/29/B/NZ3/01980, UMO-2014/13/B/NZ3/00405, MINIATURA 2017/01/X/NZ1/00332, UMO-2021/41/B/NZ3/02605).
2023-11-07 | GSE232067 | GEO
Project description:Production of morphologically and physiologically variable seeds is an important strategy that helps plants to survive in unpredictable natural conditions. However, the model plant Arabidopsis thaliana and most agronomically essential crops yield visually homogenous seeds. Using automated phenotype analysis, we observed that in Arabidopsis small seeds tend to have higher primary and secondary dormancy levels when compared to large ones. Transcriptomic analysis revealed distinct gene expression profiles between large and small seeds. Large seeds had higher expression of translation-related genes implicated in germination competence. In contrast, small seeds showed elevated expression of many positive regulators of dormancy, including a key regulator of this process – the DOG1 gene. Differences in DOG1 expression were associated with differential production of its alternative cleavage and polyadenylation isoforms where in small seeds proximal poly(A) site is selected resulting in a short mRNA isoform. Furthermore, single-seed RNA-seq analysis demonstrated that large seeds resemble DOG1 knockout mutant seeds. Finally, on the single seed level, the expression of genes affected by seed size was correlated with the expression of genes positioning seeds on the path towards germination. Our results demonstrate an unexpected link between seed size and dormancy phenotypes in a species producing highly homogenous seed pools, suggesting that the correlation between seed morphology and physiology is more widespread than initially assumed. Funded by the National Science Centre Grant, Poland UMO-2021/41/B/NZ3/02605.
2023-10-16 | GSE203310 | GEO
Project description:The DXO family proteins, highly conserved among eukaryotes, are involved in mRNA 5’-end quality control (5’QC) and also remove non-canonical NAD+ cap from RNA (deNADding). DXO1 protein, the only DXO homolog in Arabidopsis, due to its plant-specific features has a strong deNADding enzymatic activity but no apparent role in 5’ QC. Despite its significant contribution to plant RNA metabolism, a direct role for DXO1 enzymatic activity in the cell has not yet been demonstrated. Notably, all molecular and morphological changes observed so far in dxo1 mutant plants depended on the plant-specific N-terminal domain of the protein. Our investigation into the role of DXO1 in response to biotic stress, specifically its susceptibility to Pseudomonas syringae pv. tomato (Pst) DC3000 infection, unexpectedly revealed the importance of DXO1 enzymatic activity in the plant immune response. We observed that the dxo1-2 knockout mutant and transgenic dxo1-2 lines expressing a DXO1 variant either catalytically inactive (DXO1(E394A/D396A)) or lacking the N-terminal domain (DXO1(deltaN194)) exhibited enhanced resistance to Pst, accompanied by marked changes in the expression of key pathogenesis markers. Also, other markers of plant immunity, such as callose deposition and production of reactive oxygen species, were strongly induced by two pathogen effectors, elf18 and flg22, in the tested mutant lines. These results strongly suggest that both DXO1 features, the N-terminal domain and its catalytic center, contribute to the regulation of plant immunity. In conclusion, this is the first observation revealing the involvement of the DXO1 enzymatic activity in plant physiology. Founded by National Science Centre Grant, Poland (UMO-2014/13/B/NZ3/00405, UMO-2018/29/B/NZ3/01980, UMO-2021/40/Q/NZ1/00014).
2025-09-29 | GSE291552 | GEO