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Transcriptomic profile at single cell level of adult lungs expressing exogenous BCL2 driven by three mesenchymal cell-specific drivers

ABSTRACT: A transient mesenchymal cell in the lung called alveolar myofibroblast (AMF) plays a key role in alveolar formation during postnatal development. However, AMFs are cleared from the alveoli upon lung maturation. We induce exogenous BCL2 expression in these cells using three lineage-specific drivers (Myh11-CreER, PdgfraCreER, and PdgfrbCreER) to prevent AMF clearance. We use scRNA seq to analyze the transcriptomic changes of AMFs upon BCL2 expression and subsequent persistence in the lung. Additionally, we evaluated the consequences driven by persistent AMFs in other cell lineages at a transcriptional level.

ORGANISM(S): Mus musculus

PROVIDER: GSE297319 | GEO | 2025/06/25

REPOSITORIES: GEO

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