Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

MiR-302 regulates pancreatic progenitor pool and pancreatic size [mRNA-seq]

ABSTRACT: Disruptions in pancreatic development lead to health issues such as pancreatic agenesis and congenital diabetes mellitus. Understanding pancreatic organogenesis is critical for identifying disease mechanisms and developing regenerative therapies. The pancreas consists of endocrine and exocrine cells, both of which are derived from multipotent progenitor cells (MPCs). MPC proliferation and differentiation are tightly controlled by multiple mechanisms, including post-transcriptional regulation by miRNAs. However, these regulatory factors are not fully understood. Here, we profiled miRNA expression in MPCs and identified that miR-302 was highly enriched during the earliest stages of pancreatic development. Loss of miR-302 resulted in reduced pancreatic size without altering the proportions of endocrine and exocrine cells at E17.5, suggesting that miR-302 regulates MPC number rather than differentiation. Transcriptomic analysis at E10.5 revealed that miR-302 modulates genes involved in the Wnt signaling pathway and cell cycle progression. Notably, miR-302 prolonged S phase in MPCs, leading to slower cell proliferation and a smaller MPC pool at E10.5. These findings provide the first comprehensive miRNA profile during early pancreatic development and establish miR-302 as a critical regulator of MPC number and pancreas size.

ORGANISM(S): Mus musculus

PROVIDER: GSE297841 | GEO | 2025/12/18

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

MiR-302 regulates pancreatic progenitor pool and pancreatic size [miRNA-seq]

Project description:Disruptions in pancreatic development lead to health issues such as pancreatic agenesis and congenital diabetes mellitus. Understanding pancreatic organogenesis is critical for identifying disease mechanisms and developing regenerative therapies. The pancreas consists of endocrine and exocrine cells, both of which are derived from multipotent progenitor cells (MPCs). MPC proliferation and differentiation are tightly controlled by multiple mechanisms, including post-transcriptional regulation by miRNAs. However, these regulatory factors are not fully understood. Here, we profiled miRNA expression in MPCs and identified that miR-302 was highly enriched during the earliest stages of pancreatic development. Loss of miR-302 resulted in reduced pancreatic size without altering the proportions of endocrine and exocrine cells at E17.5, suggesting that miR-302 regulates MPC number rather than differentiation. Transcriptomic analysis at E10.5 revealed that miR-302 modulates genes involved in the Wnt signaling pathway and cell cycle progression. Notably, miR-302 prolonged S phase in MPCs, leading to slower cell proliferation and a smaller MPC pool at E10.5. These findings provide the first comprehensive miRNA profile during early pancreatic development and establish miR-302 as a critical regulator of MPC number and pancreas size.

2025-12-18 | GSE297840 | GEO

Comparative gene expression of mouse Pancreatic specific transcription factor 1a (Ptf1a/p48) in pancreatic progenitor cells

Project description:Ptf1a was identified as the essential transcription factor which controls pancreatic exocrine enzyme expression. With lineage tracing eperiments Ptf1a was recognized as an important pancreatic progenitor transcription factor and Ptf1a null mice do not develop a pancreas. We used gene expression arrays to determine the global differeences in expression levels when pancreatic progenitor cells are expanding in Ptf1a heterozygote versus null mutants at E10.5. Ptf1a E10.5 dorsal pancreas total RNA from pools of 3 embryos was twice linear amplified and hybridized to Affymetrix GeneChip Mouse Genome 430 2.0 in triplicate for the Ptf1a KO and in duplicate for the Ptf1a heterozygote

2011-01-24 | E-GEOD-26816 | biostudies-arrayexpress

Age-associated DNA methylation increases in mouse pancreatic islets suggest an epigenetic drift toward the exocrine pancreas epigenome

Project description:Early postnatal overnutrition causes persistent dysregulation of endocrine pancreas function. We used genome-scale DNA methylation profiling in the suckling-period small litter (SL) mouse model to test whether this occurs via persistent epigenetic changes in pancreatic islets. Although SL islets showed DNA methylation changes directly after weaning and in adulthood, few of these were present at both ages, contrary to our hypothesis. Most interestingly, we discovered that genomic regions that are hypermethylated in exocrine relative to endocrine pancreas tend to gain methylation in islets during aging. Focusing on a subset of genes relevant to β cell function, we showed that these methylation differences are strongly correlated with expression. Together, our results provide the novel insight that DNA methylation changes that occur as islets age indicate an overall epigenetic drift toward the exocrine pancreas epigenome. These concerted shifts in the islet methylome could contribute to the age-associated decline in endocrine pancreas function. Pancreatic islets were isolated from P21/P180 SL or C mice. To ensure purity of islets, 3 rounds of manual picking were performed in each samples. Whole pancreas samples, ~98% of which is exocrine pancreas, were used as exocrine pancreas. There are 5 mice per group.

2014-12-03 | E-GEOD-63811 | biostudies-arrayexpress

Comparative gene expression of mouse Pancreatic specific transcription factor 1a (Ptf1a/p48) in pancreatic progenitor cells

2011-01-24 | GSE26816 | GEO

Pancreas agenesis mutations disrupt a lead enhancer controlling a developmental enhancer cluster

Project description:Single cell RNA-Seq and ATAC-Seq in mouse E10.5 multipotent pancreatic progenitors (MPCs) and single cell ATAC-Seq of mouse E13.5 pancreas.

2022-06-30 | GSE183673 | GEO

Ptf1a-dependent gene expression during pancreas development (TLDA part)

Project description:We first used RNA-Seq technology to explore gene expression in mouse Ptf1a^YFP/+ [het] FACS sorted cells at E11.5 (early pancreatic Multipotent Progenitor Cells) and E15.5 (nascent acinar cells) as well as in Ptf1a^YFP/YFP [null] at E11.5 (delayed early MPC): this is deposited with accession number E-MTAB-449. We then examined 376 selected genes identified as differentially expressed between early pancreatic MPC and nascent acinar cells or between early pancreatic and delayed early MPCs by Taqman Low Density Arrays (TLDAs) with Real Time RT-PCR for each 1-day time point from E10.5 to E15. 5 in Ptf1a^YFP/+ [het] and for E10.5 and E11.5 in Ptf1aYFP/YFP [null] . Finally, 94 genes identified in the first phase of TLDAs (including 2 endogenous control, Gapdh and 18S) were analyzed in a second TLDA phase for each 1-day time point from E10.5 to -E18.5 in Ptf1a^YFP/+ [het] and for E11.5 in Ptf1aYFP/YFP [null] with biological replicates (n>=3) for each time point.

2014-11-15 | E-MTAB-2780 | biostudies-arrayexpress

RNA-Seq of mouse genotypes Ptf1a^YFP/+ and Ptf1a^YFP/YFP

Project description:This experiment used RNA-Seq technology to explore gene expression in mouse Ptf1a^YFP/+ [het] FACS sorted cells at E11.5 (early pancreatic Multipotent Progenitor Cells) and E15.5 (nascent acinar cells) as well as in Ptf1a^YFP/YFP [null] at E11.5 (delayed early MPC). 376 selected genes identified as differentially expressed between early pancreatic MPC and nascent acinar cells or between early pancreatic and delayed early MPCs have then been examined by Taqman Low Density Arrays (TLDAs) with Real Time RT-PCR for each 1-day time point from E10.5 to E15. 5 in Ptf1a^YFP/+ [het] and for E10.5 and E11.5 in Ptf1aYFP/YFP [null] . Finally, 94 genes identified in the first phase of TLDAs (including 2 endogenous control, Gapdh and 18S) were analyzed in a second TLDA phase for each 1-day time point from E10.5 to -E18.5 in Ptf1a^YFP/+ [het] and for E11.5 in Ptf1aYFP/YFP [null] with biological replicates (n>=3) for each time point.

2014-11-15 | E-MTAB-449 | biostudies-arrayexpress

Single-cell RNA-seq analysis of pancreatic progenitor cells and mesenchyme from mouse embryos

Project description:The pancreas is an endodermal organ with exocrine and endocrine cell compartments. The embryonic development of the pancreas relies on the the coordinated action of pancreatic progenitors and its surrounding microenvironments. The most abundant cell type of the pancreatic microenviroment are mesenchymal cells. These cells are known to contribute to the pancreas organ growth, morphogenesis and differentiation. In this study, we used single-cell RNA sequencing to molecularly characterize mesenchymal and pancreatic progenitor populations in the dorsal pancreas of the mouse embryos.

2025-10-09 | GSE283594 | GEO

High resolution spatially resolved transcriptomic analysis of mouse pancreatic sections during embryonic development

Project description:The pancreas is an endodermal organ with exocrine and endocrine cell compartments. The embryonic development of the pancreas relies on the the coordinated action of pancreatic progenitors and its surrounding microenvironments. The most abundant cell type of the pancreatic microenviroment are mesenchymal cells. These cells are known to contribute to the pancreas organ growth, morphogenesis and differentiation. In this study, we used hybridization-based in situ sequencing (HybISS) to spatially characterize pancreatic progenitor populations and microenviroment cell types in the pancreas of the mouse embryos.

2025-10-09 | GSE290835 | GEO

A single cell atlas of the murine pancreatic ductal tree identifies novel cell populations with potential implications in pancreas regeneration and exocrine pathogenesis.

Project description:Pancreatic ducts form an intricate network of tubules that secrete bicarbonate and drive acinar secretions into the duodenum. This network is formed by centroacinar cells, terminal, intercalated, intracalated ducts, and the main pancreatic duct. Ductal heterogeneity at the single-cell level has been poorly characterized. Here, we used scRNA-seq to comprehensively characterize mouse ductal heterogeneity at single-cell resolution of the entire ductal epithelium from centroacinar cells to the main duct. Moreover, we used organoid cultures, injury models and pancreatic tumor samples to interrogate the role of novel ductal populations in pancreas regeneration and exocrine pathogenesis. In our study, we have identified the coexistence of 15 ductal populations within the healthy pancreas and characterized their organoid formation capacity and endocrine differentiation potential. Cluster isolation and subsequent culturing let us identify ductal cell populations with high organoid formation capacity and endocrine and exocrine differentiation potential in vitro, including Wnt-responsive-population, ciliated-population and FLRT3+ cells. Moreover, we have characterized the location of these novel ductal populations in healthy pancreas, chronic pancreatitis and tumor samples, hightlihgting a putative role of WNT-responsive, IFN-responsive and EMT-populations in pancreatic exocrine pathogenesis as their expression inceases in chronic pancreatitis and PanIN lesions. In light of our discovery of previously unidentified ductal populations, we unmask the potential roles of specific ductal populations in pancreas regeneration and exocrine pathogenesis.

2024-03-24 | GSE262292 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data