Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

M6A-seq profiling in Mettl3 Ctrl and dTAG-13 treated mESCs

ABSTRACT: dTAG-13 treated METTL3_FKBP12(F36V) is sufficient to functionally deplete m6A methylation catalysed by METTL3/14 complex.

ORGANISM(S): Mus musculus

PROVIDER: GSE297888 | GEO | 2025/06/19

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

m6A-seq profiling in Mettl3 Ctrl and dTAG13 mESCs [m6Aseq]

Project description:dTAG-13 treated METTL3_FKBP12(F36V) is sufficient to functionally deplete m6A methylation, that catalysed by METTL3/14 complex.

2020-09-15 | GSE154707 | GEO

m6A-seq profiling in Mettl3 Ctrl and dTAG-13 treated mESCs

Project description:m6A-seq profiling in Mettl3 Ctrl and dTAG-13 treated mESCs

| PRJNA1266678 | ENA

Depletion of m6A methylation by conditional Mettl3 knockout in Ascl1-induced neurons

Project description:We have perturbed RNA methylation machinery and investigated the change in subcellular localization of mRNA in Ascl1-induced neurons (iNeurons). Mutant iNeuron line harbouring tagged endogenous Mettl3 was generated which allowed for the selective and targeted depletion of Mettl3 protein with the addition of dTAG. Cells were treated with dTAG for 40 hours then separated into compartments (neurites and soma) and then sequenced in parallel with samples which received no dTAG treatment.

2023-07-12 | E-MTAB-9643 | biostudies-arrayexpress

RNA exonuclease REXO4 resolves m6A-marked R-loops and suppresses anti-tumor immunity [RNAseq in REXO4-dTAG HeLa cells]

Project description:To determine if REXO4 depletion directly induces R-loop accumulation independent of transcription, we generated a HeLa cell line expressing endogenous REXO4 tagged with an N-terminal HA-FKBP12/F36V degron (REXO4-dTAG) via microhomology-mediated end joining (MMEJ)-based CRISPR/Cas9. Acute REXO4 degradation, achieved by a 4-hr treatment with dTAG-13 (dTAG), was then analyzed by RNA-seq to assess transcriptomic changes

2026-05-28 | GSE313849 | GEO

Proteomic raw data for Mettl3 Ctrl and RKO mice

Project description:The purpose of this project is to explore the changes in signaling pathways after Mettl3 knockout, and to identify potential downstream target proteins of Mettl3 along with the signaling pathways they are involved in. Ultimately elucidating the molecular mechanisms underlying Mettl3 pathogenesis. Retina of control and Mettl3 knockout mice were collected, and samples were then used for proteomic analysis. After tissue collection, a series of cutting-edge technologies, including protein extraction, enzyme digestion, liquid chromatography-tandem mass spectrometry, and bioinformatics analysis, were employed to investigate the quantitative proteome of the samples.

2024-10-12 | PXD056750 | Pride

m6A-seq profiling in Mettl3 Ctrl and dTAG13 mESCs [m6Aseq]

Project description:m6A-seq profiling in Mettl3 Ctrl and dTAG13 mESCs [m6Aseq]

| PRJNA647278 | ENA

The METTL3 RNA methyltransferase regulates estrogen signalling in MCF-7 breast cancer cells

Project description:siRNAs targeting METTL3 were used to deplete METTL3 in MCF-7 breast cancer cells and the effect on basal and estrogen regulated gene expression determined.

2025-11-30 | GSE195532 | GEO

m6A-seq on chromatin-assoicated RNA (ChrMeRIP-seq) in mouse embryonic stem cell [ChrMeRIP-seq]

Project description:N6-methyladenosines (m6A) are stoichiometrically deposited on exons of nearly one third of RNA Pol II transcriptome, mainly catalysed by METTL3/14 complex. However, neither the intronic methylation pattern and its functional relevance nor the immediate response upon m6A loss have been fully understood. Here we applied MeRIP-seq on mESC nascent transcriptome and thus revealed that approximately 6-10% of m6A peaks occurred at intronic regions, preferentially the conserved and alternative exon/intron part of longer introns, proximately to 5’-splice sites. These intronic m6A deposition correlates with both Rbm15 binding and H3K36me3. Moreover, coupled 4sU-seq with METTL3 dTAG system in mESC, we found that m6A mediates alternative exon/intron inclusive in nascent transcriptome. Intriguingly, m6A self-regulation including writers and readers are early response and partially contributed by alternative splicing changes. Collectively, our study presents a unified model that m6A mediates alternative splicing, and dTAG METTL3 opens an avenue to interrogate the direct response of functional m6A disruption.

2020-09-15 | GSE154706 | GEO

N6-Methyladenosine mediates alternative intron/exon inclusion in the nascent transcriptome [4sU-seq]

2020-09-15 | GSE154705 | GEO

The METTL3 RNA methyltransferase regulates androgen signalling in castrate resistant prostate cancer cells

Project description:siRNAs targeting METTL3 used to deplete METTL3 in castrate resistant LNCaP:C42 and 22Rv1 prosate cancer cells and the effect on basal and androgen regulated gene expression and splicing determined.

2022-11-02 | GSE194420 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

Tweets

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data