Dataset Information

The symbiotic system of ATP translocase-expressing bacteria (ATEB) and macrophages.

ABSTRACT: The endosymbiosis theory indicates that mitochondria evolved from free-living prokaryotes, which entered host cells and remained as endosymbionts. Here we have designed an Escherichia coli - macrophage symbiosis system. By engineering and modifying Escherichia coli to enter cells through membrane fusion, it can bypass lysosomes to avoid being degraded by cells and exist stably in macrophages. Moreover, it has been genetically engineered to express ATP. This symbiotic bacterium can serve as a mitochondrial substitute to provide the energy necessary for the metabolism of life activities of host macrophages. By up-regulating the genes related to tricarboxylic acid cycle and oxidative phosphorylation and down-regulating the genes related to glycolysis, it significantly enhances the metabolic activity of host macrophages. This symbiotic system can still exist within 60 hours and can still maintain certain functionality in the microenvironment of tumor nutritional deficiency.

ORGANISM(S): Mus musculus

PROVIDER: GSE297978 | GEO | 2026/01/01

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Animal-algal photosymbioses are a unique group of symbiotic relationships in which animals harbor photosynthetic algae within their cells and tissues. Both marine and freshwater sponges host algal endosymbionts. In previous work, we demonstrated that freshwater sponges can acquire these endosymbionts horizontally through algal infection and that potentially conserved evolutionary pathways may lead to the establishment of the endosymbioses including those involved in endocytosis, ion transport, vesicle-mediated transport, innate immunity, redox regulation, and metabolic processes. Here, we show that algal symbionts can be transferred vertically from algal-bearing overwintering gemmules to adult sponges, and that their proliferation is enhanced by light. Sponges grown under light conditions harbored higher algal loads than those in the dark; however, algae were still able to proliferate and persist in sponges reared in the dark, occupying similar spatial locations to those grown in light. RNA-Seq analysis of algal-bearing sponges across developmental stages in light and dark conditions revealed genetic regulatory pathways involved in the transmission and establishment of the endosymbiosis. Differential expression analysis indicated that the endocytosis and SNARE pathways regulate the internalization and transport of algae at the earliest stage of hatching under light conditions and later in development under dark conditions, potentially contributing to the recruitment of endosymbiotic algae. In sponges hatched in the dark, genes involved in vesicle acidification are regulated, alongside changes in the expression of genes in the pentose phosphate pathway - a key metabolic route involved in redox homeostasis and circadian rhythm regulation via NADPH metabolism, is observed. E. muelleri serves as a versatile model system, supported by robust genomic and transcriptomic resources, for studying host-symbiont interactions. It offers a unique opportunity to investigate the molecular signaling and environmental factors that shape symbiosis in a system where the host can exist with or without algal endosymbionts, symbionts can be acquired either horizontally or vertically, and proliferation of the algae can occur with or without photosynthesis.

Dataset Information

The symbiotic system of ATP translocase-expressing bacteria (ATEB) and macrophages.

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets