Single-cell RNA sequencing (scRNA-Seq) analysis of tumor-infiltrating lymphocytes (TILs) from B16-F0 melanoma in mice

ABSTRACT: To comprehensively analyze the influences on the tumor infiltrated immune cells after deficiency of APPL2 specifically in Tregs, we employed single-cell RNA sequencing (scRNAseq) to profile CD45+ TILs from both Foxp3YFP-Cre Appl2f/f and Foxp3YFP-Cre control mice. To our surprise, tumor infiltrated B cells were dramatically decreased in Foxp3YFP-Cre Appl2f/f mice. Hereby, there was a trend of increase for most of the other immune subsets. These data indicated that the infiltrated B cells, as a whole, were tumor supportive and restricting protumoral B cell infiltration was critical for Appl2-KO Tregs in suppressing tumor growth.

ORGANISM(S): Mus musculus

PROVIDER: GSE298324 | GEO | 2026/01/28

REPOSITORIES: GEO