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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE

ABSTRACT: This SuperSeries is composed of the SubSeries listed below.

ORGANISM(S): synthetic construct Homo sapiens

PROVIDER: GSE298535 | GEO | 2025/10/20

REPOSITORIES: GEO

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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [Digenome-seq]

Project description:To compare the sensitivity of CHANGE-seq-BE to another leading off-target discovery method, we performed Digenome-seq on the same ABE and CBE targets and evaluated them with matching analysis parameters. Many low-frequency off-target sites with editing rates were not identified by Digenome-seq and were only discovered by CHANGE-seq-BE.

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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [CHANGE-seq-BE for CBE targets]

Project description:To adapt and apply for cytosone base editors (CBEs), we perfomed CHANGE-seq-BE using eA3A-BE3 to target B2M, CBLB, CD7, CIITA and PDCD1 regions at different loci. CHANGE-seq-BE identified low frequency off-target editing rates ranging from 1.7% to 7.5% that are not identified by Digenome-seq further implies CHANGE-seq-BE is highly sensitive and can identify true off-targets while requiring 20-fold fewer sequencing reads.

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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [CHANGE-seq-BE for ABE targets]

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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE [gRNA sequencing for ABE targets]

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Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE

Project description:Sensitive and unbiased genome-wide profiling of base-editor-induced off-target activity with CHANGE-seq-BE

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