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Alpha Factor arrest cell cycle synchronization time-course microarray in Galactose

ABSTRACT: Alpha Factor arrest cell cycle syncronization time-course microarray in Galactose. Time course from 0 to 40 minute.

ORGANISM(S): Saccharomyces cerevisiae

PROVIDER: GSE29892 | GEO | 2011/06/11

SECONDARY ACCESSION(S): PRJNA155337

REPOSITORIES: GEO

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Alpha Factor arrest cell cycle synchronization time-course microarray in Galactose

Project description:Alpha Factor arrest cell cycle syncronization time-course microarray in Galactose. Time course from 0 to 40 minute. Time-course experiment. Samples are hybridized onto the first timepoint.

2011-06-11 | E-GEOD-29892 | biostudies-arrayexpress

Alpha Factor arrest cell cycle synchronization time-course microarray in Glucose

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Cell cycle and G1 cyclins

Project description:This SuperSeries is composed of the following subset Series: GSE29800: Lack of G1 cyclin arrest cell cycle synchronization time-course microarray in Glucose GSE29892: Alpha Factor arrest cell cycle synchronization time-course microarray in Galactose GSE29893: Alpha Factor arrest cell cycle synchronization time-course microarray in Glucose Refer to individual Series

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Rap1 Turnover Galactose Induction Time Course

Project description:A strain harboring two copies of RAP1 is used for a competition-ChIP experiment. One copy of RAP1 is expressed from the endogenous RAP1 promoter and a c-terminal 3X FLAG epitiope tag and the other is expressed from a weakened Galactose inducible promoter and a c-terminal 9X MYC tag. Following induction by 2% galactose Rap1-Myc and Rap1-Flag levels are determined genome wide using ChIP-chip. Time Course ChIP-ChIP experiment, Rap1-Flag IP and Rap1-Myc IP. 13 Time Points (0, 10, 20, 30, 40, 50, 60, 90, 120, 150, 180, 210, 240 Minutes) 2 Biological Replicates. Total Rap1 Occupancy at times 0 and 60 minutes in the time course; 2 Biological Replicates. mRNA expression levels at times 0 and 60 minutes in the time course; 2 biological Replicates. ChIPs comparing the occupancy of Rap1 in a strain containing two copies of Rap1, one with a Flag tag and one with a Myc tag, and expressed from an identical promoter.

2012-03-05 | E-GEOD-27377 | biostudies-arrayexpress

Rap1 Competition-ChIP Galactose Induction Time Course (High Resolution)

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Response to LiCl of galactose grown cells

Project description:Wild type strain CEN.PK113-7D was grown in an aerobic batch cultivation with a start concentration of 20 g/L galactose. During exponential growth at a biomass concentration of 3 g dry weight/L LiCl was added to a concentration of 10 mM. Just before addition of LiCl (time 0) and 20, 40, 60 and 140 minutes after addition of LiCl samples were taken for transcription analysis. Lithium inhibits phosphoglucomutase whereby both galactose uptake and growth is strongly affected. Keywords = Saccharomyces galactose lithium Keywords: time-course

2003-06-23 | GSE461 | GEO

Rap1 Turnover Galactose Induction Time Course

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