Multiplexed DNA Affinity purification sequencing (multiDAP-seq) of flowering plants [Brassica oleracea]

ABSTRACT: We applied multiplexed DNA affinity purification sequencing (multiDAP-seq) to profile the binding landscapes of 360 transcription factors (TFs) across ten flowering-plant genomes. Genomic DNA fragment libraries were assayed in 96-well plates against in vitro expressed HaloTag-fused TFs, generating nearly 3,000 genome-wide binding maps. These data provide a resource for identification of TF binding sites and comparative regulatory analyses, revealing deeply conserved TF-DNA interactions alongside lineage-specific rewiring events that underpin plant diversification. We also integreated these TF binding datasets with singe nuclei RNA-seq datasets produced from multiple tissues of five plant species to investigate the roles of TFs in driving cell type-specific gene expression patterns. The associated single nuclei RNA-seq datasets were submitted under BioProject ID PRJNA1262374.

ORGANISM(S): Brassica oleracea

PROVIDER: GSE298988 | GEO | 2025/06/05

REPOSITORIES: GEO