Dataset Information

RNA-seq Analysis Reveals the Anti-inflammatory Role of hnRNPF in Diabetic kidney disease

ABSTRACT: In this study, a hyperosmotic environment was simulated using 30 mM mannitol. Human renal proximal tubular epithelial (HK-2) cells were transfected to overexpress hnRNP-F, with empty vector-transfected cells serving as the negative control (NC). RNA sequencing (RNA-seq) was performed to profile transcriptomic changes, enabling comprehensive analysis of hnRNP-F overexpression-induced alterations in differentially expressed genes (DEGs) and alternative splicing events (ASEs) under hyperosmotic stress. These data were integrated with our previously acquired dataset profiling hnRNP-F-driven DEG and ASE regulation in HK-2 cells under hyperglycemic conditions.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299230 | GEO | 2025/06/07

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:Bacteria respond to osmotic stress by a substantial increase in the intracellular osmolality, adjusting their cell turgor for altered growth conditions. Using E. coli as a model organism we demonstrate here that bacterial responses to hyperosmotic stress specifically depend on the nature of osmoticum used. We show that increasing acute hyperosmotic NaCl stress above ~1.0 Os kg-1 causes a dose-dependent K+ leak from the cell, resulting in a substantial decrease in cytosolic K+ content and a concurrent accumulation of Na+ in the cell. At the same time, isotonic sucrose or mannitol treatment (non-ionic osmotica) results in a gradual increase of the net K+ uptake. Ion flux data is consistent with growth experiments showing that bacterial growth is impaired by NaCl at the concentration resulting in a switch from net K+ uptake to efflux. Microarray experiments reveal that about 40% of up-regulated genes shared no similarity in their responses to NaCl and sucrose treatment, further suggesting specificity of osmotic adjustment in E. coli to ionic- and non-ionic osmotica The observed differences are explained by the specificity of the stress-induced changes in the membrane potential of bacterial cells highlighting the importance of voltage-gated K+ transporters for bacterial adaptation to hyperosmotic stress. Experiment Overall Design: Two biological replicates per treatment with microarray analysis using the Affymetrix GeneChip E. coli Genome array. Treatments used included: Experiment Overall Design: Control - E. coli Frag1, grown to early stationary growth phase in a mineral salts medium with 0.1% glucose at 25 C Experiment Overall Design: Sucrose hyperosmotic treatment - 1.25 M sucrose added to control culture Experiment Overall Design: for 10 min. Experiment Overall Design: NaCl hyperosmotic treatment - 1.37 M NaCl added to control culture Experiment Overall Design: For microarray data comparisons the sucrose and NaCl hyperosmotic treatment data was compared to the no treatment control data separately. The sucrose to control and NaCL to control comparison data tables are linked below.

Dataset Information

RNA-seq Analysis Reveals the Anti-inflammatory Role of hnRNPF in Diabetic kidney disease

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets