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Identifying Norrin-induced genes in bEnd.3 cells

ABSTRACT: To identify genes induced by Norrin in bEnd.3 cells (mouse brain endothelial cell line), we treated the cells with recombinant Norrin. It is known that the Norrin ligand induces beta-catenin-dependent (also known as canonical) signaling through binding to a receptor complex containing Frizzled4 (FZD4). First, we confirmed that receptor complex components, FZD4, LRP5/6, and TSPAN12, were expressed in bEnd.3 cells. Next, we observed that Norrin treatment strongly induces Axin2, a beta-catenin-dependent signaling response gene. Multiple norrin-induced DEGs were identified, including P2ry1. However, it is noted that many DEGs identified in previous scRNAseq studies on retinal vascular cells in Ndp and Tspan12 KO mice are not induced in bEnd.3. cells. This observation suggests that bEnd.3 cells are a good model system for studying signal initiation and signal transduction; however, they may not capture all aspects of cellular behavior.

ORGANISM(S): Mus musculus

PROVIDER: GSE299490 | GEO | 2026/04/11

REPOSITORIES: GEO

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