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IT-scC&T-seq streamlines scalable, parallel profiling of protein-DNA interactions in single cells

ABSTRACT: Single-cell profiling protein-chromatin interactions is often constrained by complex workflows, high cost, or dependence on specialised equipment. We present indexed tagmentation-based single-cell CUT&Tag-sequencing (IT-scC&T-seq), a modular, plate-based strategy using three-round combinatorial barcoding. IT-scC&T-seq robustly profiles histone modifications and transcription factors with high specificity and throughput, supporting simultaneous analysis of multiple samples and epitopes. Notably, it enables sensitive single-cell mapping of lamina-associated domains, low-abundance chromatin features previously difficult to resolve. Applied to adult mouse mammary gland, the method reveals cell-type-specific chromatin landscapes and lineage-regulatory dynamics. Together, IT-scC&T-seq provides a scalable, cost-effective, and broadly accessible approach for high-resolution chromatin profiling.

ORGANISM(S): Mus musculus Homo sapiens

PROVIDER: GSE299567 | GEO | 2025/06/11

REPOSITORIES: GEO

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