Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

S. erythraea R5 Fall 2002 RNA Timecourse

ABSTRACT: Saccharopolyspora erythraea NRRL2338 (wildtype) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) were grown in shake flask cultures in rich medium R5. RNA samples were harvested for each strain over 5 days. 12 h samples taken for each strain were used as their respective reference ("time zero") samples. The genomic DNA control compares NRRL2338 gDNA to NRRL2338 12 h RNA to show relative gene expression at the start of the timecourse. The 12 h RNA control compares initial gene expression between the wildtype and overproducer strains. Groups of assays that are related as part of a time series. Keywords: time_series_design

ORGANISM(S): Streptomyces coelicolor

PROVIDER: GSE2996 | GEO | 2005/07/26

SECONDARY ACCESSION(S): PRJNA104769

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Similar Datasets

S. erythraea R5 Fall 2002 RNA Timecourse

Project description:Saccharopolyspora erythraea NRRL2338 (wildtype) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) were grown in shake flask cultures in rich medium R5. RNA samples were harvested for each strain over 5 days. 12 h samples taken for each strain were used as their respective reference ("time zero") samples. The genomic DNA control compares NRRL2338 gDNA to NRRL2338 12 h RNA to show relative gene expression at the start of the timecourse. The 12 h RNA control compares initial gene expression between the wildtype and overproducer strains. Groups of assays that are related as part of a time series. Computed

2005-07-26 | E-GEOD-2996 | biostudies-arrayexpress

Transcription profiling of Saccharopolyspora erythraea NRRL2338 (wild type) and K41-135 (overproducer strain from Kosan Biosciences, KOVP) grown in shake flask cultures in rich medium R5, time series

2005-07-21 | E-SMDB-2243 | biostudies-arrayexpress

S. fradiae WT and KOS155-3C R5/No Glucose 02/09/03

Project description:RNA timecourse data for Streptomyces fradiae wildtype (ATCC19609) and overproducing strain KOS155-3C(RUS). Strains were grown at 30 C in shake flask cultures in R5 medium with no glucose. RNA samples were harvested over 5 days as tylosin was produced. 12 h RNA samples of each strain were used as the reference sample(green) within their respective timecourses. The WT gDNA vs. 12 h RNA control displays relative gene expression at the beginning of the timecourse. The 12 h RNA control compares initial RNA levels between the WT and overproducer. Groups of assays that are related as part of a time series. Keywords: time_series_design

2005-07-26 | GSE2995 | GEO

Reverse engineering of industrial pharmaceutical-producing actinomycete strains

Project description:Abstract: Transcript levels in production cultures of wildtype and classically improved strains of the actinomycete bacteria Saccharopolyspora erythraea and Streptomyces fradiae were monitored using microarrays of the sequenced actinomycete S. coelicolor. Sac. erythraea and S. fradiae synthesize the polyketide antibiotics erythromycin and tylosin, respectively, and the classically improved strains contain unknown overproduction mutations. The Sac. erythraea overproducer was found to express the entire 56-kb erythromycin gene cluster several days longer than the wildtype strain. In contrast, the S. fradiae wildtype and overproducer strains expressed the 85-kb tylosin biosynthetic gene cluster similarly, while they expressed several tens of other S. fradiae genes and S. coelicolor homologs differently, including the acyl-CoA dehydrogenase gene aco and the S. coelicolor isobutyryl-CoA mutase homolog icmA. These observations indicated that overproduction mechanisms in classically improved strains can affect both the timing and rate of antibiotic synthesis, and alter the regulation of antibiotic biosynthetic enzymes and enzymes involved in precursor metabolism. This SuperSeries is composed of the SubSeries listed below.

2006-03-08 | GSE4395 | GEO

Saccharopolyspora erythraea RNA-seq

Project description:We report the high-throughput profiling of saccharopolyspora erythraea including a industrial strain HL3168 E3 and a wild-type strain NRRL23338. The aim was to evaluate the difference in expression of sRNA predicted in silico related to secondary metabolites in Saccharopolyspora erythraea.

2013-07-16 | GSE48887 | GEO

Reverse engineering of industrial pharmaceutical-producing actinomycete strains

2006-03-08 | E-GEOD-4395 | biostudies-arrayexpress

Saccharopolyspora erythraea RNA-seq

2013-07-16 | E-GEOD-48887 | biostudies-arrayexpress

Transcription profiling of Streptomyces fradiae wild type time series (ATCC19609) and overproducing strain KOS155-3C(RUS)

2005-07-21 | E-SMDB-2231 | biostudies-arrayexpress

Transcriptome analysis of wild type and erythromycin-producing strain of S. erythraea

Project description:Erythromycin is a medically important antibiotic, biosynthesized by the actinomycete Saccharopolyspora erythraea. We used transcriptomic approach to compare whole genome expression in erythromycin high-producing strain, compared to the wild type S. erythraea strain in four stages of fermentation.

2016-06-22 | GSE65007 | GEO

K24-1/EGFP R5 RNA Timecourse

Project description:The promoter upstream from eryAI (eryAp) was cloned in front of EGFP in pIJ8660. This vector was integrated into the chromosome of K24-1, a clean host version of K41-135 (the overproducer strain obtained from Kosan Biosciences). This strain was then grown in shake flask culture in R5 medium. RNA was harvested up through three days. The 12 h RNA sample was used as the reference sample for all RNA hybridizations. A genomic DNA vs. 12 h control was also done to determine initial relative transcript expression. Groups of assays that are related as part of a time series. Keywords: time_series_design

2005-07-26 | GSE2997 | GEO

OmicsDI is part of the ELIXIR infrastructure

OmicsDI is an Elixir interoperability service. Learn more ›

OmicsDI Databases

PRIDE
PeptideAtlas
MassIVE
JPOST Repository
Physiome Model Repository

EGA
EVA
ENA
LINCS
PAXDB
Cell Collective

MetaboLights
Metabolomics Workbench
MetabolomeExpress
GNPS
BioModels
FAIRDOMHub

ArrayExpress
dbGaP
ExpressionAtlas
GEO
NODE

Information

Databases
Help
API
Contact us
Code on GitHub
Terms of use
Submit Data