Dataset Information

Investigating the molecular mechanisms underlying the therapeutic effects of Detumescence Analgesic Plaster on knee osteoarthritis through RNA sequencing technology

ABSTRACT: In this study, transcriptome sequencing was used to study the mechanism of Detumescence Analgesic Plaster (DAP) in the treatment of Knee Osteoarthritis. We identified the key pathways and key genes for the treatment of Knee Osteoarthritis by transcriptome sequencing of rat cartilage issue, and completed qPCR validation. The results showed that genes such as NDUFA5, NDUFA6, NDUFS6, and COX6A2 were enriched in the oxidative phosphorylation signaling pathway. DAP intervention significantly increased the mRNA levels of NDUFA5, NDUFA6, NDUFS6, COX6A2, and MYL3 in rat cartilage tissue, alleviating damage and injury. This study provides a new idea for the molecular mechanism of Detumescence Analgesic Plaster in the treatment of KOA.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE299712 | GEO | 2025/09/01

REPOSITORIES: GEO

ACCESS DATA

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Project description:OBJECTIVE: To observe the clinical efficacy and safety of Yiqi Yangxue formula (YQYXF) on knee osteoarthritis (KOA), and to explore the underlying therapeutic mechanism of YQYXF through endogenous differential metabolites and their related metabolic pathways.METHODS: A total of 61 KOA patients were recruited and divided into the treatment group (YQYXF, 30 cases) and the control group (celecoxib, Cxb, 31 cases). Effects of these two drugs on joint pain, swelling, erythrocyte sedimentation rate (ESR) and c-reactive protein (CRP) were observed, and their safety and adverse reactions were investigated. In animal experiments, 63 SD rats were randomly divided into normal control (NC) group, sham operation (sham) group, model (KOA) group, Cxb group, as well as low-dose (YL), medium-dose (YM), and high-dose groups of YQYXF (YH). The KOA rat model was established using a modified Hulth method. Ultra-high-performance liquid chromatography/Q Exactive HF-X Hybrid Quadrupole-Orbitrap Mass (UHPLC-QE-MS)-based metabolomics technology was used to analyze the changes of metabolites in plasma samples of rats. Comprehensive (VIP) > 1 and t-test p < 0.05 conditions were used to screen the disease biomarkers of KOA, and the underlying mechanisms of YQYXF were explored through metabolic pathway enrichment analysis. The related markers of YQYXF were further verified by ELISA (enzyme-linked immunosorbent assay).RESULTS: YQYXF can improve joint pain, swelling, range of motion, joint function, Michel Lequesen index of severity for osteoarthritis (ISOA) score, Western Ontario and McMaster Universities Osteoarthritis Index (WOMAC) score, ESR, and CRP. No apparent adverse reactions were reported. In addition, YQYXF can improve cartilage damage in KOA rats, reverse the abnormal changes of 16 different metabolites, and exert an anti-KOA effect mainly through five metabolic pathways. The levels of reactive oxygen species (ROS) and glutathione (GSH) were significantly decreased after the treatment of YQYXF.CONCLUSION: YQYXF can significantly improve the clinical symptoms of KOA patients without obvious adverse reactions. It mainly improved KOA through modulating lipid metabolism-related biomarkers, reducing lipid peroxidation and oxidative stress.

Dataset Information

Investigating the molecular mechanisms underlying the therapeutic effects of Detumescence Analgesic Plaster on knee osteoarthritis through RNA sequencing technology

Dataset's files

Similar Datasets

OmicsDI is part of the ELIXIR infrastructure

Tweets