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Human airway eptithelial cell subsets exhibit distinct transcriptional profiles in response to IFN-gamma derived from influenza specific CD8 T cells.

ABSTRACT: To evaluate the impact of IFNγ secretion by influenza-specific CD8⁺ T cells on human lung epithelial cells, we stimulated total primary human lung cells with influenza peptides in the presence or absence of an IFNγ-blocking antibody. Following stimulation, epithelial cells (CD45⁻, CD31⁻, CD326⁺) were isolated by FACS and analyzed by single-cell RNA sequencing. Three stimulation conditions were compared including unstimulated, stimulated with influenza peptides plus isotype control antibody, and stimulated with influenza peptides plus anti-IFNg antibody.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299811 | GEO | 2025/12/17

REPOSITORIES: GEO

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Project description:To investigate whether virus-specific human lung memory CD8⁺ T cells exhibit distinct transcriptional responses based on viral tropism, we stimulated total human lung cells ex vivo with peptide pools from either influenza virus (epithelial-restricted) or cytomegalovirus (CMV, broader tropism). Antigen-specific CD8⁺ T cells were identified using activation-induced markers (CD25 and CD137) and sorted for bulk RNA sequencing. Despite differences in tissue residency phenotypes, influenza- and CMV-specific CD8⁺ T cells displayed highly similar activation-induced transcriptional programs across nine donors, with shared enrichment of inflammatory and T cell signaling pathways. These findings suggest that lung memory CD8⁺ T cells mount comparable responses to antigen stimulation, regardless of viral specificity or residency profile.

2025-12-17 | GSE299807 | GEO

IFN-gamma signaling early during infection dramatically enhances antiviral programs in epithelial cells

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2015-01-01 | E-GEOD-61627 | biostudies-arrayexpress

Targeting synergetic endothelial inflammation by inhibiting NFKB and JAK-STAT pathways

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2022-08-02 | GSE209748 | GEO

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Response of peripheral blood mononuclear cells to influenza virus, human cytomegalovirus, and candida albicans derived peptide stimulation.

Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 1 healthy donor. The cells were stimulated with peptides derived from influenza virus H1 and H5, human cytomegalovirus, and candida albicans for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

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Project description:CD4 T cell responses are characterized based on a limited number of molecular markers selected from exisiting knowledge. The goal of the experiment was to assess antigenic-peptide specific T-cell responses in vitro without bias using microarrays. PBMCs were isolated from 11 young (â¤ 45 years old) and old (> 60 years old) healthy donors. The cells were stimulated with peptides derived from influenza virus for 24 hours. Unstimulated cells were cultured without the peptides for 24 hours.

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