Distinct interactions between pioneer factor Ascl1/E12a and nucleosomes drive cell fate transitions [ATAC-seq]

ABSTRACT: Understanding how pioneer transcription factors target nucleosomal DNA and initiate chromatin accessibility provides insight into the earliest events in cell fate control. We used structural, biophysical, biochemical approaches to assess how the neural pioneer factor Ascl1 heterodimerizing with E12a binds nucleosomes in vitro. The nucleosomal binding, but not free DNA binding, by Ascl1/E12 is enhanced by two types of interactions with core histones. We performed ChIP-seq and ATAC-seq to investigate how histone interaction mutants of Ascl12 (called AAA, allRs, and QA respecrively) affect its global chromatin targeting and chromatin opening.

ORGANISM(S): Homo sapiens

PROVIDER: GSE299923 | GEO | 2026/06/02

REPOSITORIES: GEO