Transcriptome-wide analysis of Sense and Natural Antisense Transcripts (NATs) in Plasmodium vivax clinical isolates from uncomplicated or complicated diseases manifestation.

ABSTRACT: We developed a custom-designed 8×60K Plasmodium vivax microarray to enable genome-wide expression profiling of both nuclear and mitochondrial transcripts from field isolates. Probe selection was guided by a combination of bioinformatic prediction and experimental validation to ensure optimal detection of parasite transcripts in clinical samples. The array features 60-mer oligonucleotide probes in situ synthesized on glass slides using Agilent technology. It includes validated probes representing 5,565 nuclear genes and 19 mitochondrial genes in sense orientation, along with 5,566 and 19 genes, respectively, in antisense orientation capturing dual-stranded expression. In most cases, multiple probes were designed in both orientations against each transcript. Validation using 14 clinical samples (including both uncomplicated and severe (Hepatic Dysfunction) cases, demonstrated a high sensitivity, with ≥ 90% transcript detection. Multiple probes targeting the same gene showed consistent expression profiles across samples. Hybridization results were further validated against previous transcriptomic data and qRT-PCR assays, confirming the array's reliability. Uniquely designed for P. vivax, this sensitive and reproducible microarray provides a powerful functional genomics platform to generate high-confidence expression data and identify differentially regulated genes for both sense and Natural Antisense transcripts (NATs) from field isolates, including both the nuclear and mitochondrial transcriptomes.

ORGANISM(S): Plasmodium vivax

PROVIDER: GSE300144 | GEO | 2026/02/12

REPOSITORIES: GEO