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Differential gene expression after gamma radiation in bis-benzimidazole-treated human glioblastoma using microarray analysis

ABSTRACT: Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE30043 | GEO | 2011/08/01

SECONDARY ACCESSION(S): PRJNA144075

REPOSITORIES: GEO

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Differential gene expression after gamma radiation in bis-benzimidazole-treated human glioblastoma using microarray analysis

Project description:Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types of samples were employed in the analysis: Control (untreated) cells, 50µM ligand-treated cells, 8.5Gy radiation-treated cells, and cells treated with 50µM ligand one hour prior to irradiation.

2011-07-31 | E-GEOD-30043 | biostudies-arrayexpress

Molecular mechanism of DNA minor groove binding ligand as a radiation modulator

2011-08-01 | GSE30044 | GEO

Molecular mechanism of DNA minor groove binding ligand as a radiation modulator

Project description:Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types of samples were employed in the analysis: Control (untreated) cells, 50µM ligand-treated cells, 2Gy radiation-treated cells, and cells treated with 50µM ligand one hour prior to 2Gy irradiation.

2011-07-31 | E-GEOD-30044 | biostudies-arrayexpress

Differential gene expression profiles induced by Hoechst dyes

Project description:Bisbenzimides, or Hoechst 33258 (H258), and its derivative Hoechst 33342 (H342) are archetypal molecules for designing minor groove binders, and widely used as tools for staining DNA and analyzing side population cells. They are supravital DNA minor groove binders with AT selectivity. H342 and H258 share similar biological effects based on the similarity of their chemical structures, but also have their unique biological effects. For example, H342, but not H258, is a potent apoptotic inducer and both H342 and H258 can induce transgene overexpression in in vitro studies. However, the molecular mechanisms by which Hoechst dyes induce apoptosis and enhance transgene overexpression are unclear. The purpose of the present study is to determine the molecular mechanisms underlying different biological effects between H342 and H258.

2011-04-16 | GSE28616 | GEO

Differential gene expression profiles induced by Hoechst dyes

2011-04-16 | E-GEOD-28616 | biostudies-arrayexpress

To study the molecular mechanism of DNA minor groove binding ligand as a radiaiton modulator

2010-11-01 | GSE22808 | GEO

Gene expression based, effect of DNA minor groove binding ligand on human cells in response to ionizing radiation

2010-11-01 | GSE18422 | GEO

Gene expression based, effect of DNA minor groove binding ligand on human cells in response to ionizing radiation

Project description:Radiation induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. The data reports the expression analysis in response to DNA minor groove binding ligand with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types if samples were employed in analysis- Control i.e. untreated cells, 50ÂµM ligand treated cells, 8.5Gy radiation treated cells, cells treated with 50ÂµM ligand one hour prior to irradiation.

2010-11-01 | E-GEOD-18422 | biostudies-arrayexpress

To study the molecular mechanism of DNA minor groove binding ligand as a radiaiton modulator

Project description:Radiation induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. The data reports the expression analysis in response to DNA minor groove binding ligand with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types if samples were employed in analysis- Control i.e. untreated cells, 50ÂµM ligand treated cells, 2Gy radiation treated cells, cells treated with 50ÂµM ligand one hour prior to 2 Gy irradiation.

2010-11-01 | E-GEOD-22808 | biostudies-arrayexpress

B-cell chronic lymphocytic leukemia side population cells

Project description:Analysis of Hoechst dye 33342-effluxing side population (SP) cells from B-CLL peripheral blood mononuclear cells.

2011-09-27 | GSE30171 | GEO