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Molecular mechanism of DNA minor groove binding ligand as a radiation modulator

ABSTRACT: Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation.

ORGANISM(S): Homo sapiens

PROVIDER: GSE30044 | GEO | 2011/08/01

SECONDARY ACCESSION(S): PRJNA144077

REPOSITORIES: GEO

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Molecular mechanism of DNA minor groove binding ligand as a radiation modulator

Project description:Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types of samples were employed in the analysis: Control (untreated) cells, 50µM ligand-treated cells, 2Gy radiation-treated cells, and cells treated with 50µM ligand one hour prior to 2Gy irradiation.

2011-07-31 | E-GEOD-30044 | biostudies-arrayexpress

Differential gene expression after gamma radiation in bis-benzimidazole-treated human glioblastoma using microarray analysis

2011-08-01 | GSE30043 | GEO

Differential gene expression after gamma radiation in bis-benzimidazole-treated human glioblastoma using microarray analysis

Project description:Radiation-induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. This study reports the expression analysis in response to DNA minor groove binding ligand (DMA-5-(4-methylpiperazin-1-yl)-2-[2’-(3,4-dimethoxyphenyl)-5`-benzimidazolyl] benzimidazole, an analogue of Hoechst 33342), with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types of samples were employed in the analysis: Control (untreated) cells, 50µM ligand-treated cells, 8.5Gy radiation-treated cells, and cells treated with 50µM ligand one hour prior to irradiation.

2011-07-31 | E-GEOD-30043 | biostudies-arrayexpress

To study the molecular mechanism of DNA minor groove binding ligand as a radiaiton modulator

2010-11-01 | GSE22808 | GEO

Gene expression based, effect of DNA minor groove binding ligand on human cells in response to ionizing radiation

2010-11-01 | GSE18422 | GEO

To study the molecular mechanism of DNA minor groove binding ligand as a radiaiton modulator

Project description:Radiation induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. The data reports the expression analysis in response to DNA minor groove binding ligand with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types if samples were employed in analysis- Control i.e. untreated cells, 50ÂµM ligand treated cells, 2Gy radiation treated cells, cells treated with 50ÂµM ligand one hour prior to 2 Gy irradiation.

2010-11-01 | E-GEOD-22808 | biostudies-arrayexpress

Gene expression based, effect of DNA minor groove binding ligand on human cells in response to ionizing radiation

Project description:Radiation induced DNA damage initiates a complex series of overlapping responses responsible for the maintenance of genome integrity. The data reports the expression analysis in response to DNA minor groove binding ligand with an emphasis on its ability to afford better protection in cells exposed to ionizing radiation. Four different types if samples were employed in analysis- Control i.e. untreated cells, 50ÂµM ligand treated cells, 8.5Gy radiation treated cells, cells treated with 50ÂµM ligand one hour prior to irradiation.

2010-11-01 | E-GEOD-18422 | biostudies-arrayexpress

B-cell chronic lymphocytic leukemia side population cells

Project description:Analysis of Hoechst dye 33342-effluxing side population (SP) cells from B-CLL peripheral blood mononuclear cells.

2011-09-27 | GSE30171 | GEO

RNA-Seq of human intracranially implanted U87MG-GFP cells as a function of distance from blood vessels

Project description:We devised a novel methodology for sorting stroma-free tumor cells according to their relative distance from BVs by injecting a vascular perfusion marker (Hoechst 33342). Briefly, post 3 weeks of intracranial implantation of U87MG-GFP cell line into striatum of NOD-SCID mice, the mice were injected i.v. with Hoechst dye. Mice sacrificed, tumors retrieved, single cell dissociated and FACS sorted to isolate cells that are either Hoechst Positive (Hh) or Hoechst Negative (Hl). RNA was extracted from the sorted cells and RNA-Sequencing performed.

2019-04-30 | E-MTAB-6881 | biostudies-arrayexpress

Effect of radiation and cigarette smoke on breast cells

Project description:Exposure to genotoxic stresses such as cosmic radiation and second-hand tobacco smoke may increase the risk of breast cancer formation. Towards an understanding of how exposure to these genotoxic agents affect breast cancer biogenesis, we have shown that treating non-tumorigenic immortalized breast MCF 10A cells with low doses (0.1 Gray) of radiation as well as cigarette smoke condensate can generate a neoplastic breast cancer phenotype. The transformed phenotype promoted increased mammosphere numbers, altered cell cycle phases, and increased invasion and motility. In addition, exclusion of Hoechst 33342 dye, a surrogate marker for increased ABC transporters, was observed, which indicates a possible increase in drug resistance. Furthermore, differential gene expression profiles were generated from the individual and combination treatment. Overall, the results indicate that when normal breast cells are exposed to low dose radiation in combination with cigarette smoke condensate a phenotype is generated that exhibits traits indicative of neoplastic transformation. Taken together, these results provide a new insight into a possible etiology for breast cancer formation in individuals exposed to cosmic radiation and second-hand smoke.

2010-06-21 | GSE21066 | GEO

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