E. coli transcriptomic response to nitrogen limitation

ABSTRACT: Stochastic fluctuation in the levels of expression of a critical factor likely causes persister formation. For example, cells with high levels of expression of the HipA toxin are tolerant of antibiotics (S Hansen et al. PLoS One 2012), as are cells with low levels of expression of Krebs cycle enzymes (Manuse S et al., PLoS Biol, 2021, Conlon BP, et al. Nat Microbiol. 2016). We therefore sought to identify the critical components whose fluctuation in expression may determine formation of persisters in a N limited cell population. We reasoned that such persister genes are likely to be found among those whose expression is most affected by nitrogen starvation. We therefore performed a transcriptome analysis of E. coli responding to nitrogen starvation.

ORGANISM(S): Escherichia coli str. K-12 substr. MG1655

PROVIDER: GSE300493 | GEO | 2026/02/28

REPOSITORIES: GEO