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MOIRA is required for clock protein and gene clustering and normal circadian rhythms in Drosophila [RNA-seq]

ABSTRACT: Circadian clocks are ~24-hour timekeepers that orchestrate daily rhythms in the expression of a multitude of genes, thereby influencing a wide array of physiological processes from sleep to metabolism. The chromatin at clock-controlled genes undergoes rhythmic modifications facilitated by the activities of histone modifiers and chromatin-remodeling proteins. However, despite its significance, this area of research remains relatively under explored. Our recent investigations unveiled that Moira, a component of ATP-dependent chromatin remodeling complex SWI/SNF complex is essential for generating ~24-h circadian rhythms in Drosophila Melanogaster. Moira functions specifically as a repressor for clock regulated genes by compacting chromatin in clock neurons. The rhythm of chromatin accessibility is completely abolished in the absence of Moira. Further, Moira is organized into ~10 positionally static, discrete nuclear foci at the inner nuclear envelope across all Drosophila cells, and clock regulated genes are localized to one of these Moira foci throughout the circadian cycle specifically in the clock neurons. Our study will lead to a significant advancement in our understanding of how SWI/SNF complexes regulate chromatin structure and subnuclear localization of clock-regulated genes, and ultimately affect circadian rhythms.

ORGANISM(S): Drosophila melanogaster

PROVIDER: GSE300582 | GEO | 2025/12/01

REPOSITORIES: GEO

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MOIRA is required for clock protein and gene clustering and normal circadian rhythms in Drosophila [ATAC-seq]

Project description:Circadian clocks are ~24-hour timekeepers that orchestrate daily rhythms in the expression of a multitude of genes, thereby influencing a wide array of physiological processes from sleep to metabolism. The chromatin at clock-controlled genes undergoes rhythmic modifications facilitated by the activities of histone modifiers and chromatin-remodeling proteins. However, despite its significance, this area of research remains relatively under explored. Our recent investigations unveiled that Moira, a component of ATP-dependent chromatin remodeling complex SWI/SNF complex is essential for generating ~24-h circadian rhythms in Drosophila Melanogaster. Moira functions specifically as a repressor for clock regulated genes by compacting chromatin in clock neurons. The rhythm of chromatin accessibility is completely abolished in the absence of Moira. Further, Moira is organized into ~10 positionally static, discrete nuclear foci at the inner nuclear envelope across all Drosophila cells, and clock regulated genes are localized to one of these Moira foci throughout the circadian cycle specifically in the clock neurons. Our study will lead to a significant advancement in our understanding of how SWI/SNF complexes regulate chromatin structure and subnuclear localization of clock-regulated genes, and ultimately affect circadian rhythms.

2025-12-01 | GSE300583 | GEO

Deletion of SWI/SNF ATPase activity in hepatocytes of adult liver disrupts circadian gene expression and central liver functions [RNAseq]

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Deletion of SWI/SNF ATPase activity in hepatocytes of adult liver disrupts circadian gene expression and central liver functions [ChIPseq]

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PBAF/cBAF reorganization on H3.3 chromatin regulates BMAL1 activity in the absence of circadian negative feedback.

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Reorganization of PBAF/cBAF remodelers on H3.3 chromatin regulates BMAL1 activity in the absence of circadian negative feedback

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2025-04-28 | PXD061408 | Pride

CRISPR screen decodes SWI/SNF chromatin remodeling complex assembly

2025-04-28 | PXD054392 | Pride

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2025-05-27 | PXD043839 | Pride

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