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Long non-coding RNA AFAP1-AS1 promotes alternative splicing of AXIN2 by facilitating SRSFs phase separation to induce drug resistance in lung adenocarcinoma

ABSTRACT: In this study, we identified that the long non-coding RNA actin filament associated protein 1 antisense RNA 1 (AFAP1-AS1), which is highly expressed in lung adenocarcinoma, regulates RNA alternative splicing by recruiting splicing factors such as serine and arginine rich splicing factor 1 and 3 (SRSF1 and SRSF3), promoting their liquid-liquid phase separation. To investigate the effect of AFAP1-AS1 on alternative splicing, we overexpressed and knocked down AFAP1-AS1 in lung adenocarcinoma cell lines and obtained all regulated alternative splicing events through RNA seq, exploring the potential mechanism of AFAP1-AS1 promoting the occurrence and development of lung adenocarcinoma.

ORGANISM(S): Homo sapiens

PROVIDER: GSE300659 | GEO | 2025/12/04

REPOSITORIES: GEO

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Long non-coding RNA AFAP1-AS1 promotes alternative splicing of AXIN2 by facilitating SRSFs phase separation to induce drug resistance in lung adenocarcinoma

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