Browse
Submit Data
Databases
API
Help

Dataset Information

0 Views

0 Connections

0 Citations

0 Reanalyses

0 Downloads

Omics score: 0

Transcriptional alterations in the brain using Eomes-Cre mouse line control animals at embryonic day 16.5 - WT only

ABSTRACT: During cerebral cortex development, neural stem cells (NSCs) require precise spatiotemporal signals to acquire the correct cell identity at the exact moment. Even though epigenetic regulation plays a central role in cell fate decisions, the exact mechanisms remain elusive. Thus, we focus our research on the H3K79 methyltransferase DOT1L, a key player in cell fate decisions. Our group has shown that DOT1L prevents NSC premature differentiation by increasing expression of genes that regulate asymmetric cell division (Franz et al. 2019). Using cell-lineage tracing and pharmacological inhibition, we confirmed that when DOT1L is inhibited apical progenitors (APs) switch to symmetric neurogenic divisions in detriment of asymmetric self-renewal (Appiah et al. 2023). However, the impact on basal progenitors (BPs), responsible for the evolutionary expansion of the cortex in both size and complexity (Nonaka-Kinoshita et al, 2013), is still unexplored. As DOT1L generally preserves NSC transcriptional programs of cells dividing multiple times, the restricted proliferation potential in BPs might bear potentially novel insights into how stem cells control the tight balance between proliferation and differentiation. We now analyse control (WT) mice for DOT1L using Eomes-Cre line during cortical neurogenesis (E16.5) using single cell RNA-seq to understand how the transcriptome changes.

ORGANISM(S): Mus musculus

PROVIDER: GSE300678 | GEO | 2025/07/30

REPOSITORIES: GEO

ACCESS DATA

Json Xml

Dataset's files

Source:

			Action	DRS
		Other

Items per page:

1 - 1 of 1

Similar Datasets

Transcriptional study of the brain using Eomes-Cre mouse line control animals at embryonic day 14.5 - WT only

Project description:During cerebral cortex development, neural stem cells (NSCs) require precise spatiotemporal signals to acquire the correct cell identity at the exact moment. Even though epigenetic regulation plays a central role in cell fate decisions, the exact mechanisms remain elusive. Thus, we focus our research on the H3K79 methyltransferase DOT1L, a key player in cell fate decisions. Our group has shown that DOT1L prevents NSC premature differentiation by increasing expression of genes that regulate asymmetric cell division (Franz et al. 2019). Using cell-lineage tracing and pharmacological inhibition, we confirmed that when DOT1L is inhibited apical progenitors (APs) switch to symmetric neurogenic divisions in detriment of asymmetric self-renewal (Appiah et al. 2023). However, the impact on basal progenitors (BPs), responsible for the evolutionary expansion of the cortex in both size and complexity (Nonaka-Kinoshita et al, 2013), is still unexplored. As DOT1L generally preserves NSC transcriptional programs of cells dividing multiple times, the restricted proliferation potential in BPs might bear potentially novel insights into how stem cells control the tight balance between proliferation and differentiation. We now analyse control (WT) mice for DOT1L using Eomes-Cre line during cortical mid-neurogenesis (E14.5) using single cell RNA-seq to understand how the transcriptome changes.

2025-07-30 | GSE300648 | GEO

Transcriptional alterations in the brain following Dot1l conditional knockout using the Emx1-Cre mouse line at embryonic day 16.5 [EmxDotRNAE16]

Project description:During cerebral cortex development, neural stem cells (NSCs) require precise spatiotemporal signals to acquire the correct cell identity at the exact moment. Even though epigenetic regulation plays a central role in cell fate decisions, the exact mechanisms remain elusive. Thus, we focus our research on the H3K79 methyltransferase DOT1L, a key player in cell fate decisions. Our group has shown that DOT1L prevents NSC premature differentiation by increasing expression of genes that regulate asymmetric cell division (Franz et al. 2019). Using cell-lineage tracing and pharmacological inhibition, we confirmed that when DOT1L is inhibited apical progenitors (APs) switch to symmetric neurogenic divisions in detriment of asymmetric self-renewal (Appiah et al. 2023). We now analyse conditional knockout (cKO) and control (WT) mice for DOT1L using Emx1-Cre line during cortical mid-neurogenesis (E14.5) using single cell RNA-seq to understand how the transcriptome changes.

2025-07-30 | GSE291845 | GEO

Study of gene networks in basal progenitors during cortical neurogenesis

Project description:During cortical development neurons are generated sequentially from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activaed cell sorting (FACS) to isolate BPs from Tbr2GFP knockin reporter mice (Arnold SJ et al. Genesis, 2009) at early (embryonic day, E13) and late (embryonic day, E16) stages of cortical neurogenesis and determined mRNA expression profiles using mouse mRNA microarray (Illumina MouseWG-6 v2). Comparison of E13 and E16 mRNA expression profiles allowed us to identify regulatory gene networks for maintaining stage specific homeostasis of BPs throughout neurogenesis. FACS isolated BPs at E13 and E16 mouse brain cortex were used for microarray analyses. Six biological replicates (embryonic cortex from three different litters) for E13 and five biological replicates (embryonic cortex from three different litters) for E16 were analysed.

2014-05-01 | E-GEOD-45450 | biostudies-arrayexpress

Study of miRNAs function in basal progenitors during cortical neurogenesis

Project description:During cortical development neurons are generated sequentially from basal progenitors (BPs) which specifically express the transcription factor Tbr2. We used fluorescent-activaed cell sorting (FACS) to isolate BPs from Tbr2GFP knockin reporter mice (Arnold SJ et al. Genesis, 2009) at early (embryonic day, E13) and late (embryonic day, E16) stages of cortical neurogenesis and determined miRNA expression profiles using mouse miRNA microarray (Agilent).Comparison of E13 and E16 microRNA expression profiles allowed us to identify regulatory mechanisms for maintaining stage specific homeostasis of BPs. FACS isolated BPs at E13 and E16 mouse brain cortex were used for miRNA microarray analyses. Four biological replicates (embryonic cortex from three different litters) for each group (E13 or E16) were analysed.

2014-05-01 | E-GEOD-45449 | biostudies-arrayexpress

Study of miRNAs function in basal progenitors during cortical neurogenesis

2014-05-01 | GSE45449 | GEO

Expression profiling of MDA-MB-231 breast cancer cells treated with DOT1L inhibitors and siDOT1L

Project description:MDA-MB-231 cell line with relatively high DOT1L levels was treated with two potent, selective inhibitors of the DOT1L histone methyl transferase. These compounds can inhibit cells migration and invasion and induce differentiation. Here we provide expression profiling data of cells treated with two DOT1L inhibitors [1] [2], DOT1L siRNA (siDOT1L) or control. MDA-MB-231 cells were treated with 1uM compound [1], 5uM compound [1], 2uM compound [2], 10 uM compound [2] (DOT1L inhibitors) or control (0.1% DMSO) for 14 days, or siDOT1L for 7 days. For each unique condition, 2 biological replicates were generated for expression profiling. Compound [1]: EPZ004777 (in Diagle et al., 2011) Compound [2]: Compound 55 (in Anglin et al., 2012)

2015-02-18 | E-GEOD-56630 | biostudies-arrayexpress

Study of gene networks in basal progenitors during cortical neurogenesis

2014-05-01 | GSE45450 | GEO

SeaIce, Espegrend, Microscosm, Mnemi

Project description:454 dataset for Hosia, et al. Torstensson et al. Dinasquet et al.

| PRJEB7907 | ENA

Project description:Manavski et al

| PRJNA1192076 | ENA

Project description:Finn Et al.

| PRJNA433383 | ENA