A streamlined and comprehensive protocol for the generation and multi-omic analysis of human monocyte-derived macrophages

ABSTRACT: Macrophages are versatile immune cells with phenotypes ranging from inflammatory (M1-like) to anti-inflammatory (M2-like), shaped by diverse differentiation and polarization conditions. We present a standardized protocol to generate and characterize human macrophages from frozen PBMC-derived monocytes using cytokine profiling, transcriptomics, and snRNA-seq, including depolarization studies. This streamlined approach facilitates reproducible macrophage studies from biobanked samples, advancing both research and therapeutic applications.

ORGANISM(S): Homo sapiens

PROVIDER: GSE300681 | GEO | 2025/11/03

REPOSITORIES: GEO