Global effects on gene and retroelement expression upon HUSH function abrogation in ERVK3-1 knockout cells

ABSTRACT: ERVK3-1 is a ubiquitously expressed 109-amino acid endogenous retroviral Rec microprotein. We hereby report its physical and functional interaction with the HUSH complex via PPHLN1. Deletion of the ERVK3-1 coding sequence in HEK293T cells resulted in significant upregulation of ZNF genes and pseudogenes - established classes of HUSH targets. Increased expression of genes involved in immune recognition and signaling - especially the MAGE family - was also noted, consistent with the current knowledge on HUSH depletion increasing L1 expression and consequently, interferon signaling. Analysis of ERV expression from the same RNA-Seq dataset shows widespread dysregulation as well.

ORGANISM(S): Homo sapiens

PROVIDER: GSE300870 | GEO | 2025/06/30

REPOSITORIES: GEO