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Influence of temporal interference electrical stimulation on mRNA expression in primary rat progenitor cells in vitro.

ABSTRACT: Electrical stimulation has been shown to modulate the proliferation and differentiation of neural progenitor cells (NPCs). Although stimulation therapies, such as deep brain stimulation (DBS) have been widely used in the clinic, the mechanisms underlying clinical efficacy remain unknown. DBS via temporally interfering (TI) electric fields has emerged as a promising strategy for non-invasive stimulation (Grossman et al., 2017). However, its effect on neural development remains unexplored. Here, the influence of TI stimulation on gene expression of primary embryonic rat NPCs was investigated in vitro. We previously demonstrated that TI stimulation at the theta frequency induces increases cellular metabolism and neural differentiation at the protein level. Transcriptome analysis using mRNA-seq is carried out to investigate changes in gene expression following stimulation, identifying the activation of the MAPK/ERK pathway and changes in the extracellular matrix in the stimulated condition from GO enrichment analyses.

ORGANISM(S): Rattus norvegicus

PROVIDER: GSE300971 | GEO | 2026/06/28

REPOSITORIES: GEO

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