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Transcriptome analysis of mural granulosa cells and cumulus cells during ovulation in mouse ovary

ABSTRACT: This study investigates the transcriptomic changes in mural granulosa cells and cumulus cells collected at different time points during the ovulation process in mice following hCG stimulation. RNA-seq was performed to identify dynamic gene expression patterns associated with ovulation, and the data serve as a resource for understanding temporal regulation of ovarian function.

ORGANISM(S): Mus musculus

PROVIDER: GSE301074 | GEO | 2026/01/12

REPOSITORIES: GEO

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Rhesus monkey cumulus cells revert to a mural granulosa cell state following an ovulatory stimulus

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Project description:The granulosa cells in the mammalian ovarian follicle respond to gonadotropin signalling and are involved in the processes of folliculogenesis and oocyte maturation. Studies on gene expression and regulation in human granulosa cells are of interest due to their potential for estimating the oocyte viability and IVF success. However, the post-transcriptional gene expression studies on miRNA level in the human ovary have been scarce. The current study determined the miRNA profile by deep sequencing of the two intrafollicular somatic cell types: mural and cumulus granulosa cells isolated from women undergoing controlled ovarian stimulation and in vitro fertilization. Paired cumulus and mural granulosa samples were analysed from 3 women participating in IVF procedure. Libraries of all 6 samples were sequenced twice, generating 2 technical replicates for each sample. Differential gene expression study was performed on the pooled results of technical replicates.

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High-throughput RNA sequencing of human preovulatory cumulus and mural granulosa cells (mRNA)

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