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A novel role of BPCs in the control of medial domain differentiation during gynoecium development in Arabidopsis thaliana

ABSTRACT: The gynoecium, a highly specialized structure in flowering plants, ensures their high reproductive success through the control of different crucial steps spanning from ovule protection to fertilization and seed maturation and dispersion. Multiple bpc mutants show reduced vigor, small fruit size and height, a reduced number of seeds and problems in septum fusion and formation. BPCs are known to be involved in the regulation of key factors involved in plant development, and they are thought to function both as activators and repressors of target gene expression. Here we showed that gynoecium development is affected in different multiple mutants of the Basic PentaCysteine (BPC) genes, where the septum fails to develop properly, and that BPCs of class I and II regulate the expression of different genes involved in carpel development and phytohormonal pathways regulation. Considering the fundamental role of the gynoecium, which affects the reproductive success of the plants, we focused on understanding which genes could be putative direct targets of BPCs and thus involved in gynoecium development. We demonstrated that SPATULA and NO TRANSMITTING TRACT (NTT), which play pivotal roles in carpel and transmitting tract development, are downregulated. As a consequence, bpc multiple mutants fail to properly develop the septum and the transmitting tract. Interestingly, among the downregulated genes, we also found PIN-LIKES3, whose promoter can be directly bound by BPCs, which is an auxin efflux carrier that regulates and controls cytoplasmatic availability of auxin and could contribute also to various growth processes.

ORGANISM(S): Arabidopsis thaliana

PROVIDER: GSE301158 | GEO | 2025/12/31

REPOSITORIES: GEO

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Project description:Background: The carpel margin meristem is a vital multi-potent structure located in the medial domain of the Arabidopsis thaliana gynoecium, the female floral reproductive organ. The carpel margin meristem generates ovules that upon fertilization become seeds. The molecular mechanisms that specify this meristematic region and regulate its organogenic potential are poorly understood. Here, we present an analysis of the transcriptional profile of the medial domain of the Arabidopsis gynoecium highlighting the developmental stages that immediately proceed ovule initiation, the earliest stages of seed development. Results: Using a floral synchronization system and a SHATTERPROOF2 domain-specific reporter construct, paired with fluorescence-activated cell sorting, we assayed the transcriptome of the gynoecial medial domain with temporal and spatial precision. Our analysis reveals a set of genes that are differentially-expressed within the SHATTERPROOF2 expression domain that marks portions of the developing medial domain. Many members of this gene set have been shown previously to function during the development of medial domain-derived structures, including the ovules, thus validating our approach. Other uncharacterized members of this gene set, including a set of differentially-expressed cis-natural antisense transcripts, are potential novel regulators of medial domain development and candidates for future functional studies. Several members of the REM family of transcriptional regulators were enriched in the SHP2-expressing cell population including a previously unrecognized REM family member (At5g60142). Analysis of the abundance of specific transcriptional isoforms identified genes that may exhibit âisoform switchingâ behavior during gynoecial development. Conclusions: This data set provides genome-wide transcriptional insight into the development of the gynoecial medial domain that contains the carpel margin meristem, an important reproductive structure that gives rise to the ovules in Arabidopsis thaliana. Four samples (YFP-positive, YFP-negative, all-sorted, and non-sorted). Four biological replicates of each sample (except YFP-positive, collected in triplicated). Strand-specific libraries were sequenced (single-end) in two lanes of a HiSeq 2500 Illumina flow cell (each lane was later analyzed as a technical replicate).

Project description:Background: The carpel margin meristem is a vital multi-potent structure located in the medial domain of the Arabidopsis thaliana gynoecium, the female floral reproductive organ. The carpel margin meristem generates ovules that upon fertilization become seeds. The molecular mechanisms that specify this meristematic region and regulate its organogenic potential are poorly understood. Here, we present an analysis of the transcriptional profile of the medial domain of the Arabidopsis gynoecium highlighting the developmental stages that immediately proceed ovule initiation, the earliest stages of seed development. Results: Using a floral synchronization system and a SHATTERPROOF2 domain-specific reporter construct, paired with fluorescence-activated cell sorting, we assayed the transcriptome of the gynoecial medial domain with temporal and spatial precision. Our analysis reveals a set of genes that are differentially-expressed within the SHATTERPROOF2 expression domain that marks portions of the developing medial domain. Many members of this gene set have been shown previously to function during the development of medial domain-derived structures, including the ovules, thus validating our approach. Other uncharacterized members of this gene set, including a set of differentially-expressed cis-natural antisense transcripts, are potential novel regulators of medial domain development and candidates for future functional studies. Several members of the REM family of transcriptional regulators were enriched in the SHP2-expressing cell population including a previously unrecognized REM family member (At5g60142). Analysis of the abundance of specific transcriptional isoforms identified genes that may exhibit “isoform switching” behavior during gynoecial development. Conclusions: This data set provides genome-wide transcriptional insight into the development of the gynoecial medial domain that contains the carpel margin meristem, an important reproductive structure that gives rise to the ovules in Arabidopsis thaliana.

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A novel role of BPCs in the control of medial domain differentiation during gynoecium development in Arabidopsis thaliana

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