Silencing the Storm: A Next-Generation Secretome (PRS CK STORM) Rewrites the Immune Response in Viral Acute Respiratory Distress Syndrome

ABSTRACT: PRS CK STORM is a complex biological drug composed of the secretome derived from co-cultures of alternatively activated M2 macrophages and adipose-derived mesenchymal stem cells (ASCs). The secretome encompasses the total set of proteins and bioactive molecules released into the extracellular space, including cytokines, chemokines, growth factors, enzymes and exosomes. Rather than representing a simple mixture of isolated components, PRS CK STORM captures the physiological complexity of immunological crosstalk induced by exposing these cell types to a controlled stress environment—an approach designed to activate regenerative immune programs through dynamic intercellular communication. This drug has demonstrated therapeutic efficacy in vivo by reducing lung inflammation, viral load, and tissue injury in murine models of acute respiratory distress syndrome (ARDS). In parallel, multi-omic mechanistic assays have shown that PRS CK STORM consistently inhibits key pro-inflammatory signaling pathways including NF-κB, NLRP3 inflammasome, and COX-2 activity, supporting its immunoregulatory mechanism of action. To assess whether the molecular composition of PRS CK STORM reflects its observed immunomodulatory effects, we profiled the exosomal microRNA (miRNA) content in five independent drug production batches, enabling the identification of the most abundant miRNAs likely to contribute to its biological activity.

ORGANISM(S): Homo sapiens

PROVIDER: GSE301236 | GEO | 2026/06/30

REPOSITORIES: GEO