Developing a T>A Base Editor via Protein Universe Mining and optimizing translesion polymerase

ABSTRACT: A engineering Pol η variants offers the exciting prospect of further tailoring its activity for base editing. Improving its processivity could ensure that Pol η completes the bypass of the AP site and perhaps extends a few more bases before dissociating, thus preventing the recruitment of other, potentially less desirable, cellular polymerases or repair factors. Base on this hypothesis, We decide to develop a novel T-to-A base editor.

ORGANISM(S): Homo sapiens

PROVIDER: GSE301258 | GEO | 2025/07/08

REPOSITORIES: GEO